Fox R M, Mynderse J F, Goulian M
Biochemistry. 1977 Oct 4;16(20):4470-7. doi: 10.1021/bi00639a022.
In the presence of diethylaminoethyldextran cultured human lymphocytes will utilize deoxynucleotides for the synthesis of DNA, whereas in the absence of diethylaminoethyldextran no incorporation of deoxynucleotides is detected. Labeled deoxynucleoside mono-, di-, and triphosphates are incorporated into DNA at approximately the same rate. Deoxynucleotide incorporation is essentially linear for 10 min but continues at a gradually diminishing rate for an additional 20 to 50 min. The initial rate of DNA synthesis is at least 20 to 40% of the in vivo rate, and in those cells that are in S phase 0.7-1.5% of the DNA is synthesized. By the three properties examined (restriction to S phase, semiconservative mode, and initial product in short chains), DNA synthesis in diethylaminoethyldextran-treated cells resembles DNA synthesis in vivo.
在存在二乙氨基乙基葡聚糖的情况下，培养的人淋巴细胞会利用脱氧核苷酸来合成DNA，而在不存在二乙氨基乙基葡聚糖的情况下，则检测不到脱氧核苷酸的掺入。标记的脱氧核苷单磷酸、二磷酸和三磷酸以大致相同的速率掺入DNA。脱氧核苷酸掺入在10分钟内基本呈线性，但在接下来的20至50分钟内以逐渐降低的速率继续。DNA合成的初始速率至少是体内速率的20%至40%，并且在处于S期的那些细胞中，0.7 - 1.5%的DNA被合成。通过所检测的三个特性（限制在S期、半保留模式和短链中的初始产物），经二乙氨基乙基葡聚糖处理的细胞中的DNA合成类似于体内的DNA合成。
