Engström W, Wanger L, Sundqvist K G
Scand J Immunol. 1984 Jan;19(1):79-84. doi: 10.1111/j.1365-3083.1984.tb00902.x.
The effects of contact with a solid surface (in the form of adhesive microcarrier beads (Cytodex)) on the induction of proliferation of human blood lymphocytes after exposure to concanavalin A (Con A) were investigated. The duration of the lag phase preceding DNA synthesis was identical in the presence and absence of beads. The kinetic course of initiation of DNA synthesis was exponential but with different rate constants both in the presence and in the absence of beads during the first 4 days of the culture period. Cytodex exerted its effect by increasing the proportion of cells initiating DNA synthesis in response to Con A. To elucidate whether lymphocytes responded to Con A exposure in a dose-dependent manner, cells were seeded at different concentrations and exposed to Con A for different times. At 36 and 48 h after start of stimulation the proportion of cells synthesizing DNA increased with increasing cell number in the cultures. In contrast, the portion of cells synthesizing DNA was markedly decreased at high cell densities 60, 72 and 96 h after start of stimulation. In the presence of microcarrier beads, however, the proportion of cells synthesizing DNA was proportional to the cell number in the cultures also after 60, 72 and 96 h.
研究了与固体表面（以粘性微载体珠（Cytodex）的形式）接触对人血淋巴细胞在暴露于伴刀豆球蛋白A（Con A）后增殖诱导的影响。在有珠和无珠的情况下，DNA合成之前的延迟期持续时间相同。在培养期的前4天，无论有无珠子，DNA合成起始的动力学过程均呈指数形式，但速率常数不同。Cytodex通过增加响应Con A启动DNA合成的细胞比例发挥其作用。为了阐明淋巴细胞是否以剂量依赖方式对Con A暴露作出反应，将细胞以不同浓度接种并暴露于Con A不同时间。在刺激开始后36和48小时，培养物中合成DNA的细胞比例随细胞数量增加而增加。相反，在刺激开始后60、72和96小时，在高细胞密度下合成DNA的细胞比例明显降低。然而，在存在微载体珠的情况下，在60、72和96小时后，合成DNA的细胞比例也与培养物中的细胞数量成比例。
