犬红细胞系统：一种研究血红蛋白合成调控机制的新模型。

The canine erythroid cell system: a new model for study of regulatory mechanism in hemoglobin synthesis.

作者信息

Burka E R, Ballas S K, Rubin R N, Fischer R W, Vettore L, Winterhalter K H

出版信息

Biochim Biophys Acta. 1977 Oct 18;478(4):428-36. doi: 10.1016/0005-2787(77)90098-3.

Abstract

The total methodology for a new mammalian erythroid cell system which permits direct investigation of the terminal stages of hemoglobin synthesis and assembly is described. The canine system allows quantitative separation of native heme containing alpha and beta chains which recombine to for tetrameric hemoglobin with normal functional properties (n = 2.17). These chains can be utilized for investigation in a cell-free system in which the rates of synthesis of alpha and beta chains are equal (alpha/beta = 0.96 +/- 0.05). Methodology for the quantitative separation of globin allows study of the effects of balanced and unbalanced globin chain synthesis in both the intact cells and the cell-free system.

摘要

本文描述了一种新的哺乳动物红细胞系统的完整方法，该系统可直接研究血红蛋白合成和组装的终末阶段。犬类系统能够对含有天然血红素的α链和β链进行定量分离，这些链重新组合形成具有正常功能特性的四聚体血红蛋白（n = 2.17）。这些链可用于无细胞系统的研究，在该系统中α链和β链的合成速率相等（α/β = 0.96 +/- 0.05）。珠蛋白定量分离的方法可用于研究完整细胞和无细胞系统中平衡和不平衡珠蛋白链合成的影响。