转录/DNA修复因子TFIIH核心的第五个亚基p52的克隆与特性分析
Cloning and characterization of p52, the fifth subunit of the core of the transcription/DNA repair factor TFIIH.
作者信息
Marinoni J C, Roy R, Vermeulen W, Miniou P, Lutz Y, Weeda G, Seroz T, Gomez D M, Hoeijmakers J H, Egly J M
机构信息
Institut de Génétique et de Biologie Moléculaire et Cellulaire, Université Louis Pasteur, Strasbourg, France.
出版信息
EMBO J. 1997 Mar 3;16(5):1093-102. doi: 10.1093/emboj/16.5.1093.
Abstract
TFIIH is a multiprotein factor involved in transcription and DNA repair and is implicated in DNA repair/transcription deficiency disorders such as xeroderma pigmentosum, Cockayne syndrome and trichothiodystrophy. Eight out of the nine genes encoding the subunits forming TFIIH have already been cloned. We report here the identification, cDNA cloning and gene structure of the 52 kDa polypeptide and its homology with the yeast counterpart TFB2. This protein, along with p89/XPB, p62, p44 and p34, forms the core of TFIIH. Moreover, using in vitro reconstituted transcription and nucleotide excision repair (NER) assays and microinjection experiments, we demonstrate that p52 is directly involved in both transcription and DNA repair mechanisms in vitro and in vivo.
摘要
TFIIH是一种参与转录和DNA修复的多蛋白因子，与诸如着色性干皮病、科凯恩综合征和毛发硫营养不良等DNA修复/转录缺陷疾病有关。构成TFIIH的亚基的九个基因中有八个已经被克隆。我们在此报告52 kDa多肽的鉴定、cDNA克隆和基因结构及其与酵母对应物TFB2的同源性。这种蛋白质与p89/XPB、p62、p44和p34一起构成了TFIIH的核心。此外，通过体外重组转录和核苷酸切除修复（NER）试验以及显微注射实验，我们证明p52在体外和体内均直接参与转录和DNA修复机制。
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