Suzuki T, Horiike G, Yamazaki Y, Kawabe K, Masuda H, Miyamoto D, Matsuda M, Nishimura S I, Yamagata T, Ito T, Kida H, Kawaoka Y, Suzuki Y
Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Science, Japan.
FEBS Lett. 1997 Mar 10;404(2-3):192-6. doi: 10.1016/s0014-5793(97)00127-0.
We determined the ratio of N-glycolylneuraminic acid (Neu5Gc) to N-acetylneuraminic acid (Neu5Ac) in swine respiratory epithelia by fluorometric high-performance liquid chromatography, and examined the binding specificity of swine influenza virus strains for gangliosides containing different molecular species of sialic acid (Neu5Ac and Neu5Gc), and for bovine erythrocyte sialoglycoprotein 2 (GP-2) containing Neu5Gc as its predominate sialic acid (96% of total sialic acids). The presence of Neu5Gc, which had not been detected in human tracheal epithelia, and Neu5Ac in swine tracheal epithelia was observed in a 1:1 ratio. The swine influenza virus H1 and H3 isolates tested, except for A/swine/Iowa/15/30 (H1N1), displayed a marked binding ability for sialylsugar chains containing Neu5Gc compared with that of the human influenza virus strains. These results suggest that swine influenza viruses recognize sialylsugar chains containing the molecular species of sialic acid present predominantly in the swine tracheal epithelium.
我们通过荧光高效液相色谱法测定了猪呼吸道上皮中N-羟乙酰神经氨酸（Neu5Gc）与N-乙酰神经氨酸（Neu5Ac）的比例，并研究了猪流感病毒株对含有不同分子种类唾液酸（Neu5Ac和Neu5Gc）的神经节苷脂以及对以Neu5Gc作为主要唾液酸（占总唾液酸的96%）的牛红细胞唾液糖蛋白2（GP-2）的结合特异性。在猪气管上皮中观察到了Neu5Gc（在人气管上皮中未检测到）和Neu5Ac的存在，二者比例为1:1。除A/猪/爱荷华/15/30（H1N1）外，所检测的猪流感病毒H1和H3分离株与人类流感病毒株相比，对含有Neu5Gc的唾液糖链表现出显著的结合能力。这些结果表明，猪流感病毒识别主要存在于猪气管上皮中的唾液酸分子种类的唾液糖链。
