Kinto N, Iwamoto M, Enomoto-Iwamoto M, Noji S, Ohuchi H, Yoshioka H, Kataoka H, Wada Y, Yuhao G, Takahashi H E, Yoshiki S, Yamaguchi A
Department of Oral Pathology, School of Dentistry, Showa University, Shinagawa-ku, Tokyo, Japan.
FEBS Lett. 1997 Mar 10;404(2-3):319-23. doi: 10.1016/s0014-5793(97)00014-8.
We investigated the role of Sonic hedgehog (SHH) in osteoblast differentiation and bone formation. The numbers of ALP-positive cells in the mouse fibroblastic cell line C3H10T1/2 and the mouse osteoblastic cell line MC3T3-E1 were increased by co-culture with chicken fibroblasts transfected with chicken Shh cDNA encoding amino-terminal peptide (Shh-N). The conditioned medium of Shh-N-RCAS-transfected chicken fibroblast cultures also significantly increased ALP activity in both C3H10T1/2 and MC3T3-E1 cells. Intramuscular transplantation of Shh-N-RCAS-transfected chicken fibroblasts into athymic mice induced ectopic bone formation. These results indicate that SHH induces osteoblast differentiation and ectopic bone formation.
我们研究了音猬因子（SHH）在成骨细胞分化和骨形成中的作用。通过与转染了编码氨基末端肽的鸡Shh cDNA（Shh-N）的鸡成纤维细胞共培养，小鼠成纤维细胞系C3H10T1/2和小鼠成骨细胞系MC3T3-E1中碱性磷酸酶（ALP）阳性细胞的数量增加。转染了Shh-N-RCAS的鸡成纤维细胞培养物的条件培养基也显著提高了C3H10T1/2和MC3T3-E1细胞中的ALP活性。将转染了Shh-N-RCAS的鸡成纤维细胞肌肉内移植到无胸腺小鼠中可诱导异位骨形成。这些结果表明，SHH可诱导成骨细胞分化和异位骨形成。
