Mathieu C, Mercier A, Witt D, Debmkowski L, Tordo P
Université de Provence, C.N.R.S., U.R.A. 1412, Marseille, France.
Free Radic Biol Med. 1997;22(5):803-6. doi: 10.1016/s0891-5849(96)00424-8.
Reduction-resistant nitroxides are particularly interesting for biomedical applications. beta-Phosphorylated pyrrolidinyl nitroxides, a new series of stable pyrrolidinoxyl radicals prepared in our laboratory, were tested toward ascorbate reduction in phosphate buffer at pH 7.4. The kinetics of decay were monitored by ESR and compared to those of two reference nitroxides, PCA and Proxyl. The introduction of a beta-phosphoryl group on a pyrrolidinoxyl structure resulted in a moderate increase of the reduction rate constant. However, inside the phosphorylated series, slight structural modifications can induce significant changes in the rate constants.
抗还原氮氧化物在生物医学应用中特别有趣。β-磷酸化吡咯烷基氮氧化物是我们实验室制备的一系列新型稳定的吡咯烷基氧自由基，在pH 7.4的磷酸盐缓冲液中针对抗坏血酸盐还原进行了测试。通过电子自旋共振（ESR）监测衰变动力学，并与两种参考氮氧化物PCA和Proxyl的动力学进行比较。在吡咯烷基氧结构上引入β-磷酰基导致还原速率常数适度增加。然而，在磷酸化系列中，轻微的结构修饰可导致速率常数发生显著变化。
