Arnaud F, Goodrich R P
Cryopharm, Pasadena, Calif., USA.
Haemostasis. 1996 Mar-Apr;26(2):107-16. doi: 10.1159/000217195.
This study was designed to establish an alternative technique to the morphology scoring method described by Kunicki et al. [Transfusion 1975;15:414-421] using light transmission. A reference scale for platelet quality (expressed as a percentage of fresh cells) was established with a mixture of fresh platelets and damaged platelets frozen without cryoprotectant. The transmittance, read against autologous plasma, was compared to morphology, hypotonic stress response (HSR) and ADP-induced aggregation. The results showed that transmittance, morphology and HSR were related linearly to the percentage of fresh platelets. There was a significant correlation between relative transmittance (RT) and morphology (r = 0.932), and RT and HSR (r = 0.91), but a very poor correlation was found between transmittance and aggregation. Measurements of transmittance provided an estimation of the global aspect of a population of cryopreserved platelets in terms of shape, size and composition, and the relative transmittance of a sample to that of the fresh population could predict morphology and HSR. It is concluded that these measurements offer a valid alternative method to the subjective morphology scoring obtained with cryopreserved platelets.
本研究旨在建立一种替代技术，以取代Kunicki等人[《输血》1975年；15:414 - 421]所描述的使用光透射的形态学评分方法。通过将新鲜血小板和无冷冻保护剂冷冻的受损血小板混合，建立了血小板质量参考标准（以新鲜细胞的百分比表示）。将相对于自体血浆读取的透光率与形态学、低渗应激反应（HSR）和ADP诱导的聚集进行比较。结果表明，透光率、形态学和HSR与新鲜血小板的百分比呈线性相关。相对透光率（RT）与形态学（r = 0.932）以及RT与HSR（r = 0.91）之间存在显著相关性，但透光率与聚集之间的相关性非常差。透光率测量能够从形状、大小和组成方面对一群冷冻保存血小板的整体情况进行评估，并且样品相对于新鲜群体的相对透光率可以预测形态学和HSR。结论是，这些测量为冷冻保存血小板的主观形态学评分提供了一种有效的替代方法。
