问号钩端螺旋体内毒素中发现的一种钠钾ATP酶抑制剂的纯化与特性分析
Purification and characterization of a Na+, K+ ATPase inhibitor found in an endotoxin of Leptospira interrogans.
作者信息
Burth P, Younes-Ibrahim M, Gonçalez F H, Costa E R, Faria M V
机构信息
Departamento de Biologia Celular e Genética, Instituto de Biologia, Universidade do Estado do Rio de Janeiro, Brazil.
出版信息
Infect Immun. 1997 Apr;65(4):1557-60. doi: 10.1128/iai.65.4.1557-1560.1997.
Abstract
We showed previously that the glycolipoprotein fraction prepared from Leptospira interrogans inhibited the Na+,K+ ATPase enzyme purified from brain or kidney and in isolated nephron segments (M. Younes-Ibrahim, P. Burth, M. V. Castro Faria, B. Buffin-Meyer, S. Marsy, C. Barlet-Bas, L. Cheval, and A. Doucet, C. R. Acad. Sci. Paris Ser. III 318:619-625, 1995). In the present communication, we have demonstrated that unsaturated fatty acids such as oleic and palmitoleic acids, which are adsorbed to this fraction, are effective inhibitors of the enzyme.
摘要
我们之前曾表明，从问号钩端螺旋体制备的糖脂蛋白组分可抑制从脑或肾中纯化的Na +，K + ATP酶以及分离的肾单位节段中的该酶（M. Younes-Ibrahim，P. Burth，M. V. Castro Faria，B. Buffin-Meyer，S. Marsy，C. Barlet-Bas，L. Cheval和A. Doucet，《法国科学院院报》第III卷318:619 - 625，1995年）。在本通讯中，我们已证明吸附于该组分的不饱和脂肪酸如油酸和棕榈油酸是该酶的有效抑制剂。
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本文引用的文献
1
Colorimetric determination of blood lipides.血脂的比色测定法。
J Biol Chem. 1951 Jun;190(2):513-7.
2
THE PATHOGENESIS OF LEPTOSPIROSIS: TOXIN PRODUCTION BY LEPTOSPIRA ICTEROHAEMORRHAGIAE.钩端螺旋体病的发病机制：出血性黄疸钩端螺旋体产生毒素。
Am J Vet Res. 1964 May;25:836-43.
3
A rapid method of total lipid extraction and purification.一种快速的总脂质提取与纯化方法。
Can J Biochem Physiol. 1959 Aug;37(8):911-7. doi: 10.1139/o59-099.
4
Effects of carbazochrome sodium sulfonate (AC-17) on oleic acid-induced lung injury.
Pharmacol Toxicol. 1995 Sep;77(3):238-40. doi: 10.1111/j.1600-0773.1995.tb01019.x.
5
Pathogenesis of oleic acid-induced lung injury in the rat: distribution of oleic acid during injury and early endothelial cell changes.油酸诱导大鼠肺损伤的发病机制：损伤过程中油酸的分布及早期内皮细胞变化
Lipids. 1995 Sep;30(9):817-23. doi: 10.1007/BF02533957.
6
7
Leptospirosis.钩端螺旋体病
Clin Infect Dis. 1995 Jul;21(1):1-6; quiz 7-8. doi: 10.1093/clinids/21.1.1.
8
9
10
A potential second messenger role for unsaturated fatty acids: activation of Ca2+-dependent protein kinase.不饱和脂肪酸可能的第二信使作用：钙依赖性蛋白激酶的激活。
Science. 1984 May 11;224(4649):622-5. doi: 10.1126/science.6231726.
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