含有不同浓度水和其他成分的二棕榈酰磷脂酰胆碱单结构域样本。
Monodomain samples of dipalmitoyl phosphatidylcholine with varying concentrations of water and other ingredients.
作者信息
Powers L, Pershan P S
出版信息
Biophys J. 1977 Nov;20(2):137-52. doi: 10.1016/S0006-3495(77)85540-9.
Abstract
Methods are presented for the preparation of large monodomain phospholipid bilayer arrays containing variable amounts of water approaching the two-phase limit. The optical birefringence of these lamellar phases of dipalmitoyl phosphatidylcholine (DDPC) is measured over a range of temperature and water content, and phase transitions are observed. The techniques employed for pure DPPC and water are extended in order to produce macroscopically aligned samples containing varying concentrations of cholesterol, inorganic salts, antibiotics, and chlorophyll a. Polarization studies of the 670-nm band of chlorophyll a indicate macroscopic orientational order in the chromophore under the same conditions.
摘要
本文介绍了制备大单畴磷脂双层阵列的方法，该阵列含有接近两相极限的可变水量。在一系列温度和含水量范围内测量了二棕榈酰磷脂酰胆碱（DDPC）这些层状相的光学双折射，并观察到了相变。为了制备含有不同浓度胆固醇、无机盐、抗生素和叶绿素a的宏观排列样品，对用于纯DPPC和水的技术进行了扩展。叶绿素a 670nm波段的偏振研究表明，在相同条件下生色团中存在宏观取向有序。
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