[仓鼠胰腺细胞系（PGHAM - 1）中血型相关抗原A的意义。特别提及细胞增殖]

[The significance of blood group-related antigen A in a pancreatic cell line (PGHAM-1) in hamsters. With special reference to cell proliferation].

作者信息

Inoue M, Onda M, Uchida E

机构信息

First Department of Surgery, Nippon Medical School, Tokyo, Japan.

出版信息

Nihon Ika Daigaku Zasshi. 1997 Feb;64(1):30-8. doi: 10.1272/jnms1923.64.30.

DOI:10.1272/jnms1923.64.30

PMID:9119950

Abstract

The relationship between blood group-related antigen A and cell proliferation was studied in a pancreatic carcinoma cell line (PGHAM-1) induced by N-nitrosobis (2-oxopropyl) amine (BOP) in hamsters. In vitro, the cell proliferation of PGHAM-1 was inhibited in a culture medium with an added monoclonal antibody (MoAb) against blood group-related antigen A depending on its concentration. In in vivo experiments, immunohistochemical double staining was performed using MoAb against blood group-related antigen A and bromodeoxyuridine (BrdU) in pancreatic carcinomas by intrapancreatic transplantation of PGHAM-1. Antigen A was expressed in the cell membrane and cytoplasm of PGHAM-1 cells. The BrdU labeling index (LI) in lesions with strong expression of MoAb A (> or = 50%) was 27.4 +/- 5.00, whereas the BrdU LI in lesions with weak expression of MoAb A (< or = 10%) was 11.9 +/- 2.10. The mean LIs in strongly expressed lesions of MoAb A were higher than those in weakly expressed lesions of MoAb A (p < 0.01). These results indicated that antigen A might be associated with cell proliferation in pancreatic carcinomas induced in hamsters.

摘要

在仓鼠中，对由N-亚硝基双（2-氧代丙基）胺（BOP）诱导产生的胰腺癌细胞系（PGHAM-1），研究了血型相关抗原A与细胞增殖之间的关系。在体外，添加了抗血型相关抗原A单克隆抗体（MoAb）的培养基中，PGHAM-1的细胞增殖依其浓度受到抑制。在体内实验中，通过胰腺内移植PGHAM-1，在胰腺癌中使用抗血型相关抗原A的MoAb和溴脱氧尿苷（BrdU）进行免疫组织化学双重染色。抗原A在PGHAM-1细胞的细胞膜和细胞质中表达。MoAb A强表达（≥50%）的病变中BrdU标记指数（LI）为27.4±5.00，而MoAb A弱表达（≤10%）的病变中BrdU LI为11.9±2.10。MoAb A强表达病变中的平均LI高于MoAb A弱表达病变中的平均LI（p<0.01）。这些结果表明，抗原A可能与仓鼠诱导产生的胰腺癌中的细胞增殖有关。