Makino H, Ushijima T, Onda M, Nagao M
Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan.
Nihon Ika Daigaku Zasshi. 1997 Feb;64(1):39-44. doi: 10.1272/jnms1923.64.39.
A method was established for detecting mutations in the mouse p53 gene by cDNA-PCR-SSCP, using four cell lines which were derived from forestomach tumors induced in CDF, mice by 2-amino-3,4-dimethylimidazo [4,5-f]quinoline (MeIQ). All the cell lines were demonstrated to have mutations in exons 4, 5, 7 and 10, respectively, and the method was confirmed to be efficient and reliable. It was therefore used to analyse the role of p53 gene mutations in forestomach carcinogenesis induced by MeIQ, by examining four original tumors, one papilloma, two primary carcinomas and one lymph node metastasis. The papilloma (F 14) and the carcinoma (F 12) had mutations, but the lymph node metastasis of F 12 mouse (F 12 LN) did not. These results thus indicate that p53 mutations may occur relatively early but do not confer any predisposition for lymph node metastasis.
建立了一种通过cDNA-PCR-SSCP检测小鼠p53基因突变的方法，该方法使用了四种细胞系，这些细胞系来源于CDF小鼠经2-氨基-3,4-二甲基咪唑[4,5-f]喹啉（MeIQ）诱导产生的前胃肿瘤。所有细胞系分别在第4、5、7和10外显子中检测到突变，该方法被证实高效且可靠。因此，通过检测四个原发性肿瘤、一个乳头状瘤、两个原发性癌和一个淋巴结转移瘤，该方法被用于分析p53基因突变在MeIQ诱导的前胃癌变中的作用。乳头状瘤（F 14）和癌（F 12）存在突变，但F 12小鼠的淋巴结转移瘤（F 12 LN）没有突变。这些结果表明，p53突变可能相对较早发生，但并不赋予淋巴结转移的易感性。
