Yuki N, Tagawa Y, Irie F, Hirabayashi Y, Handa S
Department of Biochemistry, Faculty of Medicine, Tokyo Medical and Dental University, Japan.
J Neuroimmunol. 1997 Apr;74(1-2):30-4. doi: 10.1016/s0165-5728(96)00201-9.
Cumulative evidence supports the theory that anti-ganglioside antibodies function in the development of Guillain-Barré syndrome (GBS). Some patients have developed GBS after the administration of monosialoganglioside extracted from bovine brain. To clarify the pathogenesis of GBS associated with and without administration of the monosialoganglioside fraction, we investigated serum antibodies to the minor monosialogangliosides GM1b and GM1 alpha in patients with GBS and in control patients. GM1b and GM1 alpha were recognized specifically by the IgG antibody from the GBS patients. Twelve of 20 GBS patients who had high IgG anti-GM1b antibody titers had a preceding gastrointestinal infection. To evaluate the hypothesis that GM1b could be an immunogen, we determined whether a GM1b epitope was present in Campylobacter jejuni isolated from a patient with GBS associated with anti-GM1b antibody. Immunostaining with the monoclonal anti-GM1b antibody indicated that the lipopolysaccharide of the C. jejuni strain has the GM1b epitope. We speculate that an injection of bovine GM1 fraction that contains GM1b, as well as infection by an agent that bears the GM1b epitope, induces production of the anti-GM1b antibody which functions in the development of GBS in some patients.
越来越多的证据支持抗神经节苷脂抗体在吉兰-巴雷综合征（GBS）发病过程中起作用的理论。一些患者在注射从牛脑中提取的单唾液酸神经节苷脂后患上了GBS。为了阐明与注射单唾液酸神经节苷脂组分相关和无关的GBS的发病机制，我们研究了GBS患者和对照患者血清中针对次要单唾液酸神经节苷脂GM1b和GM1α的抗体。GBS患者的IgG抗体能特异性识别GM1b和GM1α。20例IgG抗GM1b抗体滴度高的GBS患者中有12例之前有胃肠道感染。为了评估GM1b可能是一种免疫原的假设，我们确定从一名患有与抗GM1b抗体相关的GBS患者分离出的空肠弯曲菌中是否存在GM1b表位。用抗GM1b单克隆抗体进行免疫染色表明，该空肠弯曲菌菌株的脂多糖具有GM1b表位。我们推测，注射含有GM1b的牛GM1组分，以及感染带有GM1b表位的病原体，会诱导抗GM1b抗体的产生，而这种抗体在一些患者的GBS发病过程中起作用。
