Dubois B, Vanbervliet B, Fayette J, Massacrier C, Van Kooten C, Brière F, Banchereau J, Caux C
Schering Plough, Laboratory for Immunological Research, Dardilly, France.
J Exp Med. 1997 Mar 3;185(5):941-51. doi: 10.1084/jem.185.5.941.
After antigen capture, dendritic cells (DC) migrate into T cell-rich areas of secondary lymphoid organs, where they induce T cell activation, that subsequently drives B cell activation. Here, we investigate whether DC, generated in vitro, can directly modulate B cell responses, using CD40L-transfected L cells as surrogate activated T cells. DC, through the production of soluble mediators, stimulated by 3- to 6-fold the proliferation and subsequent recovery of B cells. Furthermore, after CD40 ligation, DC enhanced by 30-300-fold the secretion of IgG and IgA by sIgD- B cells (essentially memory B cells). In the presence of DC, naive sIgD+ B cells produced, in response to interleukin-2, large amounts of IgM. Thus, in addition to activating naive T cells in the extrafollicular areas of secondary lymphoid organs, DC may directly modulate B cell growth and differentiation.
抗原捕获后，树突状细胞（DC）迁移至次级淋巴器官中富含T细胞的区域，在那里它们诱导T细胞活化，随后驱动B细胞活化。在此，我们使用转染了CD40L的L细胞作为替代活化T细胞，研究体外产生的DC是否能直接调节B细胞反应。DC通过产生可溶性介质，刺激B细胞增殖并使其随后恢复3至6倍。此外，CD40连接后，DC使sIgD - B细胞（主要是记忆B细胞）的IgG和IgA分泌增加30至300倍。在DC存在的情况下，幼稚sIgD + B细胞在白细胞介素 - 2的刺激下产生大量IgM。因此，除了在次级淋巴器官的滤泡外区域激活幼稚T细胞外，DC可能还直接调节B细胞的生长和分化。
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