Chandra A M, Qualls C W, Reddy G
Department of Veterinary Pathology, College of Veterinary Medicine, Oklahoma State University, Stillwater 74078, USA.
J Toxicol Environ Health. 1997 Mar;50(4):365-78. doi: 10.1080/009841097160401.
Testicular effects of TNB were characterized after single and multiple oral doses of TNB at 0, 35.5, and 71 mg/kg. Male Fischer 344 (F344) rats were killed after a single dose or after 4 and 10 daily doses of TNB. Testicular effects were not evident at the light microscope level in rats killed after a single dose of TNB or after 4 daily doses at 35.5 mg/kg of TNB. Rats receiving 4 daily doses of TNB at 71 mg/kg had the earliest evidence of testicular damage, with necrosis and degeneration of pachytene spermatocytes including a significant decrease in testicular weight. Rats dosed at 35.5 mg/kg for 10 d had severe testicular lesions, in addition to the decrease in testicular weight. There was degeneration of round and elongate spermatids, and formation of multinucleate syncytial cells. The epididymis was devoid of sperm, instead containing exfoliated syncytial spermatids. Rats dosed at 71 mg/kg of TNB for 10 d had testicular atrophy and cessation of spermatogenesis. These rats also had apoptic cells in the ventral prostate. To assess the extent of reversibility in these atrophied testis, rats were allowed to recover for 10 or 30 d after 10 doses of TNB (71 mg/kg). A significant regenerative attempt with proliferating spermatocytes were present at 10 d and elongate spermatids were evident at 30 d. These reversibility studies indicate testicular effects of TNB are at least partially reversible.
在以0、35.5和71毫克/千克的剂量单次和多次口服三硝基苯（TNB）后，对其睾丸效应进行了表征。雄性Fischer 344（F344）大鼠在单次给药后或在每日给予TNB 4次和10次后被处死。在单次给予TNB或在35.5毫克/千克的TNB每日给药4次后处死的大鼠中，光镜水平下未观察到明显的睾丸效应。接受71毫克/千克的TNB每日给药4次的大鼠最早出现睾丸损伤迹象，粗线期精母细胞出现坏死和变性，包括睾丸重量显著下降。以35.5毫克/千克的剂量给药10天的大鼠除睾丸重量下降外，还出现严重的睾丸病变。圆形和长形精子细胞发生变性，并形成多核合胞体细胞。附睾中没有精子，而是含有脱落的合胞体精子细胞。以71毫克/千克的TNB给药10天的大鼠出现睾丸萎缩和精子发生停止。这些大鼠的腹侧前列腺中也有凋亡细胞。为了评估这些萎缩睾丸的可逆程度，在给予10次TNB（71毫克/千克）后，让大鼠恢复10天或30天。在10天时可见有增殖的精母细胞进行显著的再生尝试，在30天时可见长形精子细胞。这些可逆性研究表明，TNB对睾丸的影响至少部分是可逆的。
