Chandra A M, Qualls C W, Campbell G A, Reddy G
Department of Veterinary Pathology, College of Veterinary Medicine, Oklahoma State University, Stillwater 74078, USA.
J Toxicol Environ Health. 1997 Mar;50(4):379-87.
The applicability of PCNA as a tool for the analysis of germ cells in rats treated with 1,3,5-trinitrobenzene (TNB), a potent testicular toxicant, was evaluated. Male Fischer 344 (F344) rats were gavaged with TNB at 71 mg/kg or with corn oil (vehicle). Rats were killed after 10 daily oral doses or were allowed to recover for 10 or 30 d after the 10 doses. Testes from control rats, treated rats, and rats allowed to recover were immunohistochemically stained for PCNA. PCNA labeling in the control rats was confined to the nuclei of spermatogonia, pachytene spermatocytes, and nuclei of elongate spermatocytes. Conventional (hematoxylin and eosin) staining of testes from rats treated with TNB at 71 mg/kg for 10 d revealed loss of germ cells and cessation of spermatogenesis. Immunohistochemical staining of sections from these treated rats revealed only PCNA-positive spermatogonia. Rats allowed a 10-d recovery had both spermatogonial and spermatocytic staining, indicating partial restoration of germ-cell population. In rats allowed to recover for 30 d, the PCNA staining pattern was identical to the control rats. These results indicate that PCNA can be used to assess the proliferative status of spermatogonia (germ cells) in rodent testes exposed to testicular toxicants.
评估了增殖细胞核抗原（PCNA）作为分析用强效睾丸毒物1,3,5-三硝基苯（TNB）处理的大鼠生殖细胞工具的适用性。雄性Fischer 344（F344）大鼠经口给予71 mg/kg的TNB或玉米油（赋形剂）。在每日经口给药10次后处死大鼠，或在10次给药后使其恢复10天或30天。对对照大鼠、处理过的大鼠以及恢复后的大鼠的睾丸进行PCNA免疫组织化学染色。对照大鼠中的PCNA标记局限于精原细胞、粗线期精母细胞的细胞核以及伸长型精母细胞的细胞核。用71 mg/kg的TNB处理10天的大鼠睾丸的常规（苏木精和伊红）染色显示生殖细胞丢失和精子发生停止。对这些处理过的大鼠的切片进行免疫组织化学染色仅显示PCNA阳性的精原细胞。恢复10天的大鼠既有精原细胞染色也有精母细胞染色，表明生殖细胞群部分恢复。在恢复30天的大鼠中，PCNA染色模式与对照大鼠相同。这些结果表明，PCNA可用于评估暴露于睾丸毒物的啮齿动物睾丸中精原细胞（生殖细胞）的增殖状态。
