Nabika T
Department of Laboratory Medicine, Shimane Medical University, Izumo.
Rinsho Byori. 1997 Feb;45(2):107-11.
Recent progress in positional cloning strategy has facilitated analysis of genes responsible for multifactorial diseases such as essential hypertension. To overcome heterogeneous characteristics in essential hypertension, several methods have been developed. Among these, linkage analysis using genetic rat models of hypertension is one of the most promising strategies. We performed linkage analysis using the most commonly used hypertensive model rat, the spontaneously hypertensive rat, finding 3 genomic regions on chromosomes 1, 3 and 10 containing the genes with putative responsibility for hypertension. Since a candidate SA gene was known to be located on chromosome 1, we evaluated this genetic locus in human essential hypertension. Unfortunately, the result did not support the major effects of the SA locus in human essential hypertension. However, with this strategy, we can expect that major genes responsible for hypertension will be identified in the near future.
定位克隆策略的最新进展促进了对诸如原发性高血压等多因素疾病相关基因的分析。为克服原发性高血压的异质性特征，已开发出多种方法。其中，利用高血压基因大鼠模型进行连锁分析是最具前景的策略之一。我们使用最常用的高血压模型大鼠——自发性高血压大鼠进行连锁分析，在1号、3号和10号染色体上发现了3个基因组区域，这些区域包含可能与高血压相关的基因。由于已知一个候选SA基因位于1号染色体上，我们在人类原发性高血压中评估了这个基因位点。遗憾的是，结果并不支持SA基因位点在人类原发性高血压中起主要作用。然而，通过这种策略，我们有望在不久的将来鉴定出导致高血压的主要基因。
