Holcik M, Liebhaber S A
Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia 19104, USA.
Proc Natl Acad Sci U S A. 1997 Mar 18;94(6):2410-4. doi: 10.1073/pnas.94.6.2410.
Assembly of a sequence-specific RNA-protein complex on the 3' untranslated region (3'UTR) of human alpha-globin mRNA (alpha-complex) correlates with mRNA stabilization. Here we map a limited segment of the alpha-globin 3'UTR that is both necessary and sufficient for alpha-complex formation. The sequence of this binding region identifies three additional, highly stable mRNAs that share closely related, pyrimidine-rich cis-motifs in their respective 3'UTRs. Each mRNA assembles a sequence-specific ribonucleoprotein complex at this conserved region. These complexes are structurally related, and each contains a 39-kDa cytoplasmic poly(C) binding protein previously demonstrated to be essential to formation of the alpha-complex. These observations indicate the existence of a general determinant for stabilization of eukaryotic mRNAs.
人α-珠蛋白mRNA的3'非翻译区(3'UTR)上序列特异性RNA-蛋白质复合物(α-复合物)的组装与mRNA稳定性相关。在此,我们绘制了α-珠蛋白3'UTR的一个有限片段,该片段对于α-复合物的形成既是必需的也是足够的。这个结合区域的序列鉴定出另外三种高度稳定的mRNA,它们在各自的3'UTR中共享密切相关的富含嘧啶的顺式基序。每种mRNA在这个保守区域组装一个序列特异性核糖核蛋白复合物。这些复合物在结构上相关,并且每种都包含一种39 kDa的细胞质聚(C)结合蛋白,先前已证明该蛋白对于α-复合物的形成至关重要。这些观察结果表明存在真核mRNA稳定化的一般决定因素。