一种使经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离的DNA结合蛋白复性的简单方法。 - Suppr

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超能文献

A simple method to renature DNA-binding proteins separated by SDS-polyacrylamide gel electrophoresis.

作者信息

Ossipow V, Laemmli U K, Schibler U

机构信息

Département de Biologie Moléculaire, Université de Genève, Switzerland.

出版信息

Nucleic Acids Res. 1993 Dec 25;21(25):6040-1. doi: 10.1093/nar/21.25.6040.

DOI:10.1093/nar/21.25.6040

PMID:8290367

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC310492/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6387/310492/e69f6a68d713/nar00074-0246-a.jpg

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Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes.从十二烷基硫酸钠 - 聚丙烯酰胺凝胶中洗脱蛋白质、去除十二烷基硫酸钠以及酶活性的复性：大肠杆菌RNA聚合酶σ亚基、小麦胚芽DNA拓扑异构酶及其他酶的实验结果

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