一种使经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离的DNA结合蛋白复性的简单方法。 - Suppr

Suppr

超能文献

A simple method to renature DNA-binding proteins separated by SDS-polyacrylamide gel electrophoresis.

作者信息

Ossipow V, Laemmli U K, Schibler U

机构信息

Département de Biologie Moléculaire, Université de Genève, Switzerland.

出版信息

Nucleic Acids Res. 1993 Dec 25;21(25):6040-1. doi: 10.1093/nar/21.25.6040.

DOI:10.1093/nar/21.25.6040

PMID:8290367

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC310492/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6387/310492/e69f6a68d713/nar00074-0246-a.jpg

相似文献

A simple method to renature DNA-binding proteins separated by SDS-polyacrylamide gel electrophoresis.一种使经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离的DNA结合蛋白复性的简单方法。

Nucleic Acids Res. 1993 Dec 25;21(25):6040-1. doi: 10.1093/nar/21.25.6040.

[Detection of DNA-binding proteins in polyacrylamide gel after denaturing electrophoresis].

Mol Biol (Mosk). 1992 Mar-Apr;26(2):307-14.

SDS polyacrylamide gel electrophoresis of proteins.蛋白质的十二烷基硫酸钠聚丙烯酰胺凝胶电泳

Methods Mol Biol. 1994;32:23-34. doi: 10.1385/0-89603-268-X:23.

Gradient SDS polyacrylamide gel electrophoresis of proteins.蛋白质的梯度十二烷基硫酸钠聚丙烯酰胺凝胶电泳

Methods Mol Biol. 1994;32:35-8. doi: 10.1385/0-89603-268-X:35.

Separation and analysis of membrane proteins by SDS-polyacrylamide gel electrophoresis.

Methods Mol Biol. 1993;19:203-10. doi: 10.1385/0-89603-236-1:203.

One-dimensional SDS-polyacrylamide gel electrophoresis (1D SDS-PAGE).一维十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（1D SDS-PAGE）。

Methods Enzymol. 2014;541:151-9. doi: 10.1016/B978-0-12-420119-4.00012-4.

SDS-polyacrylamide gel electrophoretic analysis of proteins in the presence of guanidinium hydrochloride.

Biotechniques. 1996 Mar;20(3):376-8. doi: 10.2144/19962003376.

Observation of orientation and relaxation of protein-sodium dodecyl sulfate complexes during pulsed intermittent field polyacrylamide gel electrophoresis.脉冲间歇场聚丙烯酰胺凝胶电泳过程中蛋白质 - 十二烷基硫酸钠复合物取向与松弛的观察

Electrophoresis. 1991 May;12(5):373-5. doi: 10.1002/elps.1150120509.

Differential silver-staining sodium dodecyl sulfate-polyacrylamide gel electrophoresis: a nonisotopic method for characterizing gel-separated histone-DNA complexes.差异银染十二烷基硫酸钠-聚丙烯酰胺凝胶电泳：一种用于表征凝胶分离的组蛋白-DNA复合物的非同位素方法。

Anal Biochem. 1998 Oct 1;263(1):79-84. doi: 10.1006/abio.1998.2775.

One-dimensional SDS gel electrophoresis of proteins.蛋白质的一维十二烷基硫酸钠凝胶电泳

Curr Protoc Protein Sci. 2001 May;Chapter 10:Unit 10.1. doi: 10.1002/0471140864.ps1001s00.

引用本文的文献

Unraveling biomolecular interactions: a comprehensive review of the electromobility shift assay.解析生物分子相互作用：电泳迁移率变动分析综述

Photochem Photobiol Sci. 2025 Sep 11. doi: 10.1007/s43630-025-00773-0.

Analysis of physicochemical properties of dry-cured beef made from Hanwoo and Holstein meat distributed in South Korea.对韩国市场上销售的韩牛和荷斯坦牛肉制成的干腌牛肉的理化性质分析。

Heliyon. 2023 Jun 12;9(6):e17091. doi: 10.1016/j.heliyon.2023.e17091. eCollection 2023 Jun.

Exosome Structures Supported by Machine Learning Can Be Used as a Promising Diagnostic Tool.由机器学习支持的外泌体结构可作为一种很有前景的诊断工具。

Materials (Basel). 2022 Nov 11;15(22):7967. doi: 10.3390/ma15227967.

Effect of Seawater on the Technological Properties of Chicken Emulsion Sausage in a Model System.海水对模型体系中鸡肉乳化香肠工艺特性的影响。

Food Sci Anim Resour. 2020 Apr;40(3):377-387. doi: 10.5851/kosfa.2020.e18. Epub 2020 Apr 30.

Polyclonal antibodies against the TLA1 protein also recognize with high specificity the D2 reaction center protein of PSII in the green alga Chlamydomonas reinhardtii.针对 TLA1 蛋白的多克隆抗体也高度特异性地识别绿藻莱茵衣藻 PSII 的 D2 反应中心蛋白。

Photosynth Res. 2012 Apr;112(1):39-47. doi: 10.1007/s11120-012-9733-x. Epub 2012 Mar 23.

Prodynorphin CpG-SNPs associated with alcohol dependence: elevated methylation in the brain of human alcoholics.原啡肽 CpG-SNPs 与酒精依赖相关：酒精成瘾者大脑中的甲基化水平升高。

Addict Biol. 2011 Jul;16(3):499-509. doi: 10.1111/j.1369-1600.2011.00323.x. Epub 2011 Apr 26.

Human NCU-G1 can function as a transcription factor and as a nuclear receptor co-activator.人类NCU-G1可作为转录因子和核受体共激活因子发挥作用。

BMC Mol Biol. 2007 Nov 16;8:106. doi: 10.1186/1471-2199-8-106.

Characterization of cis-regulatory elements and transcription factor binding: gel mobility shift assay.顺式调控元件及转录因子结合的表征：凝胶迁移率变动分析

Methods Mol Biol. 2007;366:183-201. doi: 10.1007/978-1-59745-030-0_10.

Identification of candidates for a subunit vaccine against extraintestinal pathogenic Escherichia coli.鉴定针对肠外致病性大肠杆菌的亚单位疫苗候选物。

Infect Immun. 2007 Apr;75(4):1916-25. doi: 10.1128/IAI.01269-06. Epub 2006 Dec 4.

The POU transcription factor Oct-1 represses virus-induced interferon A gene expression.POU转录因子Oct-1抑制病毒诱导的干扰素A基因表达。

Mol Cell Biol. 2005 Oct;25(19):8717-31. doi: 10.1128/MCB.25.19.8717-8731.2005.

本文引用的文献

CCAAT/enhancer-binding protein mRNA is translated into multiple proteins with different transcription activation potentials.CCAAT/增强子结合蛋白信使核糖核酸被翻译成具有不同转录激活潜能的多种蛋白质。

Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):8219-23. doi: 10.1073/pnas.90.17.8219.

Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes.从十二烷基硫酸钠 - 聚丙烯酰胺凝胶中洗脱蛋白质、去除十二烷基硫酸钠以及酶活性的复性：大肠杆菌RNA聚合酶σ亚基、小麦胚芽DNA拓扑异构酶及其他酶的实验结果

Anal Biochem. 1980 Nov 15;109(1):76-86. doi: 10.1016/0003-2697(80)90013-5.

The detection of DNA-binding proteins by protein blotting.通过蛋白质印迹法检测DNA结合蛋白。

Nucleic Acids Res. 1980 Jan 11;8(1):1-20. doi: 10.1093/nar/8.1.1.

Photochemical addition of amino acids to 14C-uracil.氨基酸向14C-尿嘧啶的光化学加成反应。

Biochem Biophys Res Commun. 1969 Feb 7;34(3):354-7. doi: 10.1016/0006-291x(69)90840-7.

Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割

Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.

A single polypeptide possesses the binding and transcription activities of the adenovirus major late transcription factor.一种单一的多肽具备腺病毒主要晚期转录因子的结合和转录活性。

Mol Cell Biol. 1986 Dec;6(12):4723-33. doi: 10.1128/mcb.6.12.4723-4733.1986.

Purification and biochemical characterization of the promoter-specific transcription factor, Sp1.启动子特异性转录因子Sp1的纯化及生化特性分析

Science. 1986 Oct 3;234(4772):47-52. doi: 10.1126/science.3529394.

The ubiquitous octamer-binding protein Oct-1 contains a POU domain with a homeo box subdomain.普遍存在的八聚体结合蛋白Oct-1包含一个带有同源异型盒亚结构域的POU结构域。

Genes Dev. 1988 Dec;2(12A):1582-99. doi: 10.1101/gad.2.12a.1582.

A cloned octamer transcription factor stimulates transcription from lymphoid-specific promoters in non-B cells.一个克隆的八聚体转录因子可刺激非B细胞中淋巴特异性启动子的转录。

Nature. 1988 Dec 8;336(6199):544-51. doi: 10.1038/336544a0.

Use of T7 RNA polymerase to direct expression of cloned genes.使用T7 RNA聚合酶指导克隆基因的表达。

Methods Enzymol. 1990;185:60-89. doi: 10.1016/0076-6879(90)85008-c.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验