Saunders D A, Beals T F, Schultz J S
Tissue Antigens. 1979 Aug;14(2):73-85. doi: 10.1111/j.1399-0039.1979.tb00826.x.
We have utilized H-2 specific antisera from congenic resistant donor recipient combinations to localize H-2 antigens in tissue sections using the indirect immunofluorescent technique. H-2 specific immunofluorescence is observed in spleen, kidney, and liver sections: however, especially with liver sections, there are cases in which the expected specific stain is weak or absent. In addition to microscopic evaluation by eye, we have employed a microscope attached photometer; recordings from this instrument differentiate positive from control stained spleen sections.
我们利用来自同源抗性供体-受体组合的H-2特异性抗血清，采用间接免疫荧光技术在组织切片中定位H-2抗原。在脾脏、肾脏和肝脏切片中观察到H-2特异性免疫荧光：然而，特别是肝脏切片，存在预期的特异性染色较弱或缺失的情况。除了通过肉眼进行显微镜评估外，我们还使用了一台连接显微镜的光度计；该仪器的记录区分了阳性和对照染色的脾脏切片。
