Fukusato T, Gerber M A, Thung S N, Ferrone S, Schaffner F
Am J Pathol. 1986 May;123(2):264-70.
Recent studies have demonstrated aberrant expression and topographical heterogeneity of HLA Class I and Class II antigens in tissues of patients with certain immunologic or neoplastic diseases. Current information about the expression of HLA antigens by normal and diseased hepatocytes is controversial. We analyzed the HLA antigenic profile of 4 normal fetal livers, 5 normal adult livers, 7 cases of chronic hepatitis B virus (HBV) infection, 14 cases of cirrhosis of various etiologies, 11 hepatic neoplasms, and 5 continuous cell lines derived from hepatic tumors. The specimens were tested by the indirect fluorescent antibody method with a panel of monoclonal antibodies to distinct monomorphic determinants of HLA Class I and Class II antigens and to beta 2-microglobulin. HLA Cells I antigens were not detected on normal fetal and adult hepatocytes, but were displayed on the plasma membrane of hepatocytes in the majority of all hepatic diseases tested and of the 5 hepatic tumor cell lines. There was a significant correlation between the expression of HLA Class I antigens on hepatocytes and the intensity of intralobular inflammation. Double immunofluorescent staining of livers infected with hepatitis B virus demonstrated simultaneous expression of HLA Class I antigens and HBsAg or HBcAg only in a small percentage of hepatocytes, suggesting lack of a specific association between HLA Class I and these viral antigens. HLA Class II antigens were not detected on hepatocytes from any of the liver diseases tested but were expressed by one of the 5 liver carcinoma cell lines analyzed. These findings confirm that HLA Class I antigens are not detectable within the limits of several immunohistochemical methods on normal hepatocytes and suggest that injury by a variety of factors directly or indirectly leads to induction of these antigens on the plasma membrane of hepatocytes.
最近的研究表明，在某些免疫性或肿瘤性疾病患者的组织中，HLA I类和II类抗原存在异常表达和拓扑异质性。目前关于正常和患病肝细胞HLA抗原表达的信息存在争议。我们分析了4例正常胎儿肝脏、5例正常成人肝脏、7例慢性乙型肝炎病毒（HBV）感染、14例各种病因的肝硬化、11例肝肿瘤以及5种源自肝肿瘤的连续细胞系的HLA抗原谱。通过间接荧光抗体法，用一组针对HLA I类和II类抗原以及β2-微球蛋白不同单态决定簇的单克隆抗体对标本进行检测。在正常胎儿和成人肝细胞上未检测到HLA I类抗原，但在所有检测的肝脏疾病的大多数肝细胞以及5种肝肿瘤细胞系的质膜上均有显示。肝细胞上HLA I类抗原的表达与小叶内炎症强度之间存在显著相关性。对感染乙型肝炎病毒的肝脏进行双重免疫荧光染色显示，仅在一小部分肝细胞中同时表达HLA I类抗原和HBsAg或HBcAg，这表明HLA I类与这些病毒抗原之间缺乏特异性关联。在所检测的任何肝脏疾病的肝细胞上均未检测到HLA II类抗原，但在所分析的5种肝癌细胞系中的一种上有表达。这些发现证实，在几种免疫组织化学方法的检测范围内，正常肝细胞上无法检测到HLA I类抗原，并表明多种因素直接或间接造成的损伤会导致这些抗原在肝细胞质膜上的诱导表达。
