Pérez-Ruiz T, Martínez-Lozano C, Tomás V, Sidrach C
Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, Spain.
Analyst. 1997 Feb;122(2):115-8. doi: 10.1039/a606841c.
The photooxidation of ascorbic acid sensitized by Thionine Blue was studied. The Leucothionine Blue formed during the reaction is highly fluorescent. A flow injection method using merging zones is proposed for the determination of ascorbic acid over a concentration range from 8 x 10(-7) to 5 x 10(-5) mol l-1 with a throughout of 80 samples per h. The method was used for the simple and rapid determination of ascorbic acid in pharmaceuticals, fruit juices and soft drinks.
研究了硫堇蓝敏化抗坏血酸的光氧化反应。反应过程中形成的无色硫堇蓝具有很强的荧光性。提出了一种使用合并带的流动注射法，用于测定浓度范围为8×10⁻⁷至5×10⁻⁵mol l⁻¹的抗坏血酸，每小时可处理80个样品。该方法用于药物、果汁和软饮料中抗坏血酸的简单快速测定。
