Jiang L, Foster F M, Ward P, Tasevski V, Luttrell B M, Conigrave A D
Department of Biochemistry, University of Sydney, New South Wales, Australia.
Biochem Biophys Res Commun. 1997 Mar 27;232(3):626-30. doi: 10.1006/bbrc.1997.6345.
Extracellular ATP and ATP gamma S (1-1000 microM) stimulated cyclic AMP (cAMP) production in undifferentiated HL-60 cells. The potency order for adenine nucleotides and adenosine was ATP gamma S > ATP > > ADP > 3 AMP = Adenosine. Indomethacin (50 microM) had no effect on ATP-induced cAMP production. ATP and ATP gamma S also suppressed cell growth and induced differentiation as revealed by fMLP-stimulated beta-glucuronidase release 48 h after exposure. The potency order for the induction of fMLP-stimulated beta-glucuronidase release by adenine nucleotides and adenosine was ATP gamma S > 3 ATP > ADP > AMP = Adenosine approximately 0. The protein kinase A inhibitor Rp-8-Br-cAMPS (10-200 mM) suppressed ATP-induced differentiation but had no effect on ATP-dependent growth suppression. UTP which, like ATP, activates P2U receptors on HL-60 cells, had no effect on cAMP production, cell growth, or differentiation. The data suggest the existence of a novel receptor for ATP on undifferentiated HL-60 cells that is coupled to the activation of adenylate cyclase and cAMP-dependent differentiation.
细胞外ATP和ATPγS（1 - 1000微摩尔）可刺激未分化的HL - 60细胞产生环磷酸腺苷（cAMP）。腺嘌呤核苷酸和腺苷的效力顺序为：ATPγS > ATP > > ADP > 3 - AMP = 腺苷。吲哚美辛（50微摩尔）对ATP诱导的cAMP产生没有影响。ATP和ATPγS还能抑制细胞生长并诱导分化，这在暴露48小时后通过fMLP刺激的β - 葡萄糖醛酸酶释放得以体现。腺嘌呤核苷酸和腺苷诱导fMLP刺激的β - 葡萄糖醛酸酶释放的效力顺序为：ATPγS > 3 - ATP > ADP > AMP = 腺苷≈0。蛋白激酶A抑制剂Rp - 8 - Br - cAMPS（10 - 200毫摩尔）可抑制ATP诱导的分化，但对ATP依赖的生长抑制没有影响。与ATP一样能激活HL - 60细胞上P2U受体的UTP，对cAMP产生、细胞生长或分化没有影响。数据表明未分化的HL - 60细胞上存在一种新的ATP受体，它与腺苷酸环化酶的激活及cAMP依赖的分化相关。
