Matthes E, Fenske H, Eichhorn I, Langen P, Lindigkeit R
Cell Differ. 1977 Oct;6(3-4):241-51. doi: 10.1016/0045-6039(77)90019-7.
The extractability of the different histone types from rat liver chromatin was studied following the incorporation of bromodeoxyuridine (BrUdR) in liver DNA. This was accomplished by a continuous application of 20 mumol BrUdR/ml/h 17--41 after partial hepatectomy. As a result, thymidine (TdR) replacement by BrUdR of about 80% in the newly-synthesized DNA strand of approx. 30% of total liver DNA was obtained; this causes remarkable changes in the histone--DNA interactions as determined from the release of histones from liver nuclei by ammonium sulfate and ethidium bromide (EB), respectively. In particular, the relative amounts of the two slightly lysine-rich histones H2A and H2B remaining on the BrUdR chromatin proved to be about 3-fold higher than those remaining on the control chromatin of TdR-treated animals. Similarly, histones H1 and H3 tend to bind closer to BrUdR-containing DNA. These results may be of interst with regard to the well-known selective effects of BrUdR on differentiation processes.
在大鼠肝脏DNA中掺入溴脱氧尿苷（BrUdR）后，研究了从大鼠肝脏染色质中提取不同组蛋白类型的能力。这是通过在部分肝切除术后17至41小时连续应用20μmol BrUdR/ml/h来实现的。结果，在约30%的总肝脏DNA的新合成DNA链中，BrUdR取代胸苷（TdR）的比例约为80%；分别通过硫酸铵和溴化乙锭（EB）从肝细胞核中释放组蛋白来确定，这导致组蛋白与DNA相互作用发生显著变化。特别是，留在BrUdR染色质上的两种略富含赖氨酸的组蛋白H2A和H2B的相对量，被证明比留在TdR处理动物的对照染色质上的相对量高约3倍。同样，组蛋白H1和H3倾向于更紧密地结合到含BrUdR的DNA上。就BrUdR对分化过程的众所周知的选择性作用而言，这些结果可能具有重要意义。
