采用高效液相色谱法测定人血浆和红细胞样本中曲唑酮及其代谢物1-间氯苯基哌嗪的含量。

Determination of trazodone and its metabolite, 1-m-chlorophenyl-piperazine, in human plasma and red blood cell samples by HPLC.

作者信息

Vatassery G T, Holden L A, Hazel D K, Dysken M W

机构信息

GRECC Program, Research Service, VA Medical Center, Minneapolis, MN 55417, USA.

出版信息

Clin Biochem. 1997 Mar;30(2):149-53. doi: 10.1016/s0009-9120(96)00166-x.

DOI:10.1016/s0009-9120(96)00166-x

PMID:9127697

Abstract

OBJECTIVES

To develop an HPLC method for the analysis of trazodone and its metabolite, 1-m-chlorophenyl-piperazine (m-CPP), in human plasma and red blood cells.

DESIGN AND METHODS

The analytes were extracted by heptane containing 1.5% isoamyl alcohol, back extracted into phosphoric acid and analyzed by reverse phase HPLC with UV detection.

RESULTS

In seven randomly selected, male, human subjects plasma concentrations (nmol/l) were 380-5841 for trazodone and 14-237 for m-CPP while those in packed red blood cells were 98-634 for trazodone and 15-155 for m-CPP. Plasma trazodone concentrations were 4-11-fold higher than those in red blood cells while those of m-CPP were about equal. This may be the first report on concentrations of trazodone and m-CPP in human red blood cells.

CONCLUSIONS

This sensitive method can be used for monitoring trazodone and m-CPP in human plasma and red blood cells.

摘要

目的

建立一种用于分析人血浆和红细胞中曲唑酮及其代谢物1-间氯苯基哌嗪（m-CPP）的高效液相色谱法。

设计与方法

分析物用含1.5%异戊醇的庚烷萃取，反萃取至磷酸中，并用紫外检测的反相高效液相色谱法进行分析。

结果

在7名随机选取的男性人体受试者中，曲唑酮的血浆浓度（nmol/l）为380 - 5841，m-CPP为14 - 237；而在红细胞中，曲唑酮为98 - 634，m-CPP为15 - 155。血浆中曲唑酮的浓度比红细胞中的高4 - 11倍，而m-CPP的浓度大致相等。这可能是关于人红细胞中曲唑酮和m-CPP浓度的首篇报道。