猪胃黏膜支链岩藻糖鞘脂的结构研究

Structural studies on branched fucosphingolipids of hog gastric mucosa.

作者信息

Slomiany B L, Slomiany A

出版信息

Chem Phys Lipids. 1977 Sep;20(1):57-69. doi: 10.1016/0009-3084(77)90055-x.

Abstract

A structural studies have been performed on new complex glycolipids extracted from hog gastric mucosa by 0.4 M sodium acetate in methanol-chloroform-water, and which were purified to homogeneity by DEAE-Sephadex and Florisil column chromatography and by preparative thin-layer chromatography in three solvent systems. Five branched fucolipids have been purified from this extract, three of which have been characterized previously [1] and remaining two were subject of this investigations. Based on the results of partial acid hydrolyses, oxidation with periodate and chromium trioxide, permethylation and serological activities following structures were proposed.

摘要

已对用0.4M醋酸钠的甲醇 - 氯仿 - 水混合液从猪胃粘膜中提取的新型复合糖脂进行了结构研究，这些糖脂通过DEAE - 葡聚糖凝胶和弗罗里硅土柱色谱法以及在三种溶剂系统中的制备薄层层析法纯化至同质。已从该提取物中纯化出五种支链岩藻糖脂，其中三种先前已被鉴定[1]，其余两种是本研究的对象。根据部分酸水解、高碘酸盐和三氧化铬氧化、全甲基化以及血清学活性的结果，提出了以下结构。