Krstulovic A M, Brown P R, Rosie D M, Champlin P B
Clin Chem. 1977 Nov;23(11):1984-8.
Concentrations of total tryptophan were determined rapidly and sensitively in 50 microliter of serum by a reversed-phase partition version of high-performance liquid chromatography. For determination of total tryptophan, sample preparation requires only precipitation of the serum protein with trichloroacetic acid and removing excess trichloroacetic acid with a 1,1,2-trichlorotrifluoroethane(Freon)/tri-N-octylamine solution. Tryptophan in serum samples was detected by ultraviolet and fluorescence spectrometry. No interferences from the naturally occurring constituents of serum were observed. Elution time for tryptophan is 15 min, the limit of detection is 1 pmol.
采用反相分配高效液相色谱法，可快速灵敏地测定50微升血清中总色氨酸的浓度。测定总色氨酸时，样品制备只需用三氯乙酸沉淀血清蛋白，并用1,1,2 - 三氯三氟乙烷（氟利昂）/三正辛胺溶液去除过量的三氯乙酸。血清样品中的色氨酸通过紫外和荧光光谱法进行检测。未观察到血清天然成分的干扰。色氨酸的洗脱时间为15分钟，检测限为1皮摩尔。
