Hervé C, Beyne P, Jamault H, Delacoux E
Service de Biochimie, Hôpital Beaujon 100, Clichy, France.
J Chromatogr B Biomed Appl. 1996 Jan 12;675(1):157-61. doi: 10.1016/0378-4347(95)00341-x.
An isocratic reversed-phase high-performance liquid chromatographic method for the simultaneous determination of tryptophan and four metabolites of the kynurenine pathway (kynurenine, 3-hydroxykynurenine, kynurenic acid and 3-hydroxyanthranilic acid) in human serum is described. This new method, which uses both isocratic elution and two on-line connected programmable ultraviolet and spectrofluorimetric detectors, allows the determination of these metabolites, in the physiological ranges, with satisfying specificity and sensitivity within 30 min.
描述了一种等度反相高效液相色谱法，用于同时测定人血清中的色氨酸和犬尿氨酸途径的四种代谢物（犬尿氨酸、3-羟基犬尿氨酸、犬尿酸和3-羟基邻氨基苯甲酸）。这种新方法采用等度洗脱以及两个在线连接的可编程紫外和荧光检测器，能够在30分钟内以令人满意的特异性和灵敏度测定生理范围内的这些代谢物。
