3N型α-2,3-唾液酸转移酶和VII型α-1,3-岩藻糖转移酶与唾液酸化路易斯（x）合成及肺癌患者生存率相关。

alpha-2,3-Sialyltransferase type 3N and alpha-1,3-fucosyltransferase type VII are related to sialyl Lewis(x) synthesis and patient survival from lung carcinoma.

作者信息

Ogawa J I, Inoue H, Koide S

机构信息

First Department of Surgery, School of Medicine, Tokai University, Japan.

出版信息

Cancer. 1997 May 1;79(9):1678-85. doi: 10.1002/(sici)1097-0142(19970501)79:9<1678::aid-cncr7>3.0.co;2-8.

DOI:10.1002/(sici)1097-0142(19970501)79:9<1678::aid-cncr7>3.0.co;2-8

PMID:9128982

Abstract

BACKGROUND

Biosynthesis of sialyl Lewis(x) (sLe(x)) requires a sialyltransferase for alpha-2,3-sialylation and a fucosyltransferase for alpha-1,3-fucosylation. To date, five human alpha-1,3-fucosyltransferase (Fuc-T) genes and five human alpha-2,3-sialyltransferase (ST) genes have been cloned. However, it is not known which enzyme is mainly responsible for sLe(x) synthesis.

METHODS

Three hundred thirteen patients with nonsmall cell lung carcinoma who had a curative tumor resection were the subjects of this study. Using tumor tissues fixed in formaldehyde, amplification of genomic DNA of Fuc-T and ST was performed by PCR and correlated with sLe(x) staining and patient prognosis.

RESULTS

The frequency of strong ST3N and Fuc-TVII amplification was significantly higher than that of STZ, ST4, Fuc-TIII, Fuc-TV, and Fuc-TVI amplification (P < 0.01). The frequency of sLe(x) staining was similar to ST3N and Fuc-TVII amplification. Survival of the patients whose tumors had strong amplification of both ST3N and Fuc-TVII was significantly shorter than that of patients whose tumors had no amplification of either gene (P < 0.01). In a multivariate analysis of survival, Fuc-TVII remained a statistically significant prognostic factor.

CONCLUSIONS

In lung carcinoma, ST3N and Fuc-TVII may both be related to sLe(x) synthesis, and Fuc-TVII is a more important indicator of poor prognosis.

摘要

背景

唾液酸化路易斯寡糖x（sLe(x)）的生物合成需要一种用于α-2,3-唾液酸化的唾液酸转移酶和一种用于α-1,3-岩藻糖基化的岩藻糖基转移酶。迄今为止，已克隆出5个人类α-1,3-岩藻糖基转移酶（Fuc-T）基因和5个人类α-2,3-唾液酸转移酶（ST）基因。然而，尚不清楚哪种酶主要负责sLe(x)的合成。

方法

本研究以313例行根治性肿瘤切除术的非小细胞肺癌患者为研究对象。利用甲醛固定的肿瘤组织，通过聚合酶链反应（PCR）对Fuc-T和ST的基因组DNA进行扩增，并与sLe(x)染色及患者预后相关联。

结果

ST3N和Fuc-TVII强扩增的频率显著高于STZ、ST4、Fuc-TIII、Fuc-TV和Fuc-TVI的扩增频率（P<0.01）。sLe(x)染色频率与ST3N和Fuc-TVII扩增相似。肿瘤同时有ST3N和Fuc-TVII强扩增的患者的生存期显著短于肿瘤这两个基因均无扩增的患者（P<0.01）。在生存期的多因素分析中，Fuc-TVII仍然是一个具有统计学意义的预后因素。