Benard G, Mendes-Giannini M J, Juvenale M, Miranda E T, Duarte A J
Labóratorio de Immunogenética e Transplante Experimental LIM-56, Faculdade de Medicina da Universidade de São Paulo (FMUSP), Brazil.
J Infect Dis. 1997 May;175(5):1263-7. doi: 10.1086/593694.
To assess human cellular immune response to paracoccidioidomycosis (PCM), lymphocyte proliferative responses to purified antigens from Paracoccidioides brasiliensis were determined in healthy persons previously infected by the fungus (positive donors), in healthy noninfected persons (controls), and in PCM patients. Affinity-purified gp70 and gp43, the two major antigens in humoral immune responses, were used. Both induced lymphocyte proliferation (gp43 species-specific) in positive donors but not in controls; healthy persons previously infected by Histoplasma capsulatum reacted to gp70 and not to gp43. A similar cross-reactivity in antibody response to gp70 was previously reported; however, antibody response to gp43 has been considered specific. Lymphocytes from PCM patients, who, unlike positive donors, have high levels of anti-gp43 and anti-gp70 antibodies, proliferated poorly with gp70 and gp43 but better with other stimuli. This dichotomy between humoral and cellular antigen-specific responses suggests a Th2 immune response in PCM, which may be related to failure to control the infection.
为评估人体对副球孢子菌病（PCM）的细胞免疫反应，我们检测了健康的真菌既往感染者（阳性供体）、健康的未感染者（对照）以及PCM患者对巴西副球孢子菌纯化抗原的淋巴细胞增殖反应。我们使用了体液免疫反应中的两种主要抗原，即亲和纯化的gp70和gp43。二者均可诱导阳性供体的淋巴细胞增殖（gp43具有种属特异性），但对照无此反应；既往感染荚膜组织胞浆菌的健康人对gp70有反应，而对gp43无反应。此前曾报道过对gp70抗体反应存在类似的交叉反应；然而，对gp43的抗体反应一直被认为具有特异性。与阳性供体不同，PCM患者体内抗gp43和抗gp70抗体水平较高，其淋巴细胞对gp70和gp43的增殖反应较差，但对其他刺激的反应较好。体液和细胞抗原特异性反应之间的这种二分法表明PCM中存在Th2免疫反应，这可能与无法控制感染有关。
