Chen H, Ke Y, Oates A J, Barraclough R, Rudland P S
Cancer and Polio Research Fund Laboratories, School of Biological Sciences, University of Liverpool, UK.
Oncogene. 1997 Apr 3;14(13):1581-8. doi: 10.1038/sj.onc.1200993.
Benign rat mammary epithelial cells transfected with restriction enzyme-fragmented DNA from a human malignant metastatic cell line (Ca2-83) produces transfectants that yield metastatic tumours in syngeneic rats. The six metastasis-inducing DNAs (Met-DNAs) that have been isolated from such transfectants are subgene in size and do not code for any expressed mRNAs, but correspond to potential regulatory regions of human DNA from malignant, metastatic cells. In pilot studies the one Met-DNA tested is detectable in some human breast tumours but not in normal tissue. Transfection of all six Met-DNAs singly into the benign mammary epithelial cells causes enhanced expression of osteopontin, whilst transfection of cDNA for osteopontin also induces the metastatic state. These results show that short regulatory DNAs exist in human cancer cells that can induce metastatic spread via a common effector gene, osteopontin, in model rat mammary cell lines.
用来自人类恶性转移细胞系（Ca2 - 83）经限制性内切酶切割的DNA转染的良性大鼠乳腺上皮细胞，产生的转染子能在同基因大鼠中形成转移性肿瘤。从这些转染子中分离出的6种转移诱导DNA（Met - DNA）在大小上属于亚基因，不编码任何表达的mRNA，但对应于来自恶性转移细胞的人类DNA的潜在调控区域。在初步研究中，所测试的一种Met - DNA在一些人类乳腺肿瘤中可检测到，但在正常组织中未检测到。将所有6种Met - DNA单独转染到良性乳腺上皮细胞中会导致骨桥蛋白表达增强，而转染骨桥蛋白的cDNA也会诱导转移状态。这些结果表明，人类癌细胞中存在短调控DNA，其可通过共同效应基因骨桥蛋白在模型大鼠乳腺细胞系中诱导转移扩散。
