Marcelo C L, Dunham W R
Department of Surgery, University of Michigan Medical School, Ann Arbor 48109-0592, USA.
J Invest Dermatol. 1997 May;108(5):758-62. doi: 10.1111/1523-1747.ep12292151.
The effect of all-trans retinoic acid on the proliferation of essential fatty acid (EFA)-deficient and of EFA-supplemented adult human keratinocytes was investigated. EFA-deficient cell strains were supplied with one of four different fatty acid-supplemented media at the P0 to P1 passage. All-trans retinoic acid at 0.5 or 1.0 microM was added to the cultures at the P1 to P2 passage. At passage P3, and 3 and 7 d thereafter, the cell growth rate was determined. The fatty acid content of cultures grown in each medium was measured using gas chromatography. All the EFA media "normalized" the cellular fatty acid composition and drastically decreased the cell number and total DNA and protein of the cultures. All-trans retinoic acid at 1 microM prevented the loss of cell viability and growth usually associated with EFA supplementation but did not affect the control (EFA deficient) or 18:1 fatty acid-supplemented cultures. All-trans retinoic acid at 1 microM altered the fatty acid content of the EFA-supplemented cultures. A statistically significant increase in 14:0, 14:1, 16:1, 18:1, and 20:4 fatty acids occurred, whereas the amounts of 18:0 and 18:2 fatty acids decreased. The largest changes were in 16:1 fatty acid (8-14%) and 18:2 fatty acid (12-5%). All-trans retinoic acid at 0.5 microM also affected both cell growth and fatty acid composition without induction of the CRABP II message. These studies demonstrate that all-trans retinoic acid stimulates the growth of EFA-supplemented keratinocyte cultures while also altering the fatty acid composition of the cells.
研究了全反式维甲酸对必需脂肪酸（EFA）缺乏型和补充了EFA的成人角质形成细胞增殖的影响。在P0至P1传代时，为EFA缺乏的细胞株提供四种不同的补充脂肪酸培养基之一。在P1至P2传代时，向培养物中添加0.5或1.0微摩尔的全反式维甲酸。在P3代以及此后的第3天和第7天，测定细胞生长速率。使用气相色谱法测量在每种培养基中生长的培养物的脂肪酸含量。所有的EFA培养基都使细胞脂肪酸组成“正常化”，并显著降低了培养物中的细胞数量、总DNA和蛋白质。1微摩尔的全反式维甲酸可防止通常与补充EFA相关的细胞活力和生长损失，但不影响对照（EFA缺乏）或补充18:1脂肪酸的培养物。1微摩尔的全反式维甲酸改变了补充EFA的培养物的脂肪酸含量。14:0、14:1、16:1、18:1和20:4脂肪酸的含量出现统计学显著增加，而18:0和18:2脂肪酸的含量则下降。最大的变化是16:1脂肪酸（8 - 14%）和18:2脂肪酸（12 - 5%）。0.5微摩尔的全反式维甲酸也影响细胞生长和脂肪酸组成，且不诱导CRABP II信息。这些研究表明，全反式维甲酸刺激补充EFA的角质形成细胞培养物的生长，同时也改变细胞的脂肪酸组成。
