Hee Chung S, Terashi H, Rhodes L M, Moon N, Dunham W R, Marcelo C L
Division of Plastic and Reconstructive Surgery, Department of Surgery, University of Michigan Medical Centers, Ann Arbor, MI 48109, USA.
J Ethnopharmacol. 2001 Jan;74(1):53-61. doi: 10.1016/s0378-8741(00)00342-1.
The effect of three different Korean Traditional Medicines (KTM) was studied on several functional parameters of adult human cells in culture. The cells were non-transformed strains of normal, skin epidermal cells (keratinocytes) from adult humans. Aqueous extracts of the herbal medicines were tested using two types of cell strains: one type was essential fatty acid deficient (EFAD) cells which grow rapidly in medium that was low in calcium and had no essential fatty acids; the second type was a cell strain grown in medium supplemented with essential fatty acid (EFA-supplemented). These cells had much slower, in vivo skin growth rates, and the fatty acid composition resembled that measured in epidermal biopsy tissue. The KTMs chosen for this study were tae-gang-hual-tang (for treating osteoarthritis), hual-ak-tang (for pain relief) and sip-zeon-tae-bo-tang (for fortifying immune systems). Because high proliferation rates usually correlate with skin inflammation and because many of the chemotactic agents mediating inflammatory response are modified fatty acids, this study focused on cell growth rate and membrane fatty acid composition as signals for the effects of the herbal medicines. By monitoring growth rate, these experiments measured both a stimulatory and a regulatory effect on the growth of keratinocytes. Some toxicity was seen at the highest doses of the KTMs. These effects were modeled mathematically, and the results showed varying effects on growth rate depending on dose and herbal recipe. The fitting parameters were discussed as they relate to biological function. The experimental design was also discussed and alternatives were suggested.
研究了三种不同的韩国传统医学药物（KTM）对培养的成人人类细胞的几个功能参数的影响。这些细胞是来自成年人的正常皮肤表皮细胞（角质形成细胞）的未转化菌株。使用两种细胞株测试草药的水提取物：一种是必需脂肪酸缺乏（EFAD）细胞，其在低钙且无必需脂肪酸的培养基中快速生长；另一种是在补充了必需脂肪酸（EFA补充）的培养基中生长的细胞株。这些细胞在体内的皮肤生长速度要慢得多，其脂肪酸组成类似于在表皮活检组织中测得的组成。本研究选择的KTM分别是太岗化汤（用于治疗骨关节炎）、化恶汤（用于缓解疼痛）和十全大补汤（用于增强免疫系统）。由于高增殖率通常与皮肤炎症相关，并且由于许多介导炎症反应的趋化剂是修饰的脂肪酸，因此本研究将细胞生长速率和膜脂肪酸组成作为草药效果的信号重点关注。通过监测生长速率，这些实验测量了对角质形成细胞生长的刺激和调节作用。在KTM的最高剂量下观察到了一些毒性。对这些效应进行了数学建模，结果表明根据剂量和草药配方，对生长速率有不同的影响。讨论了与生物学功能相关的拟合参数。还讨论了实验设计并提出了替代方案。
