Marcelo C L, Rhodes L M, Dunham W R
Department of Dermatology, University of Michigan Medical School, Ann Arbor 48109-0528.
J Invest Dermatol. 1994 Oct;103(4):564-8. doi: 10.1111/1523-1747.ep12396864.
Cultured adult human keratinocytes show accelerated growth rates in medium that is essential fatty acid deficient. The cells also show decreased amounts of the essential fatty acids 18:2, 20:3, and 20:4 and contain increased amounts of the monounsaturated fatty acids 16:1 and 18:1. These lower levels of polyunsaturated fatty acids were only partially restored by supplementing the medium with 18:2 and 20:4 fatty acid. The addition of the non-essential fatty acid 16:0 (5 microM), along with the essential fatty acids, resulted in the successful normalization of the major fatty acids in the deficient keratinocytes. Normalized cells showed a constant total fatty acid/mg of protein in the phospholipid fraction, as the total cell fatty acid content per cell increased with augmenting fatty acid supplementation. Supplementation of the medium with 16:0 and essential fatty acids decreased the growth and passage potential of the cells. Use of 18:1 in lieu of 18:2 fatty acid yielded essential-fatty-acid-deficient keratinocyte growth values. Likewise the least supplemented medium (5 microM 18:2 + 5 microM 16:0) also gave the accelerated cell growth rates. This study shows that manipulation of the essential fatty acid levels, if accompanied by 5 microM 16:0 in the growth medium, alters the growth properties of adult human primary keratinocytes.
在缺乏必需脂肪酸的培养基中，培养的成人人类角质形成细胞显示出加速的生长速率。这些细胞还显示必需脂肪酸18:2、20:3和20:4的含量降低，而单不饱和脂肪酸16:1和18:1的含量增加。通过在培养基中补充18:2和20:4脂肪酸，多不饱和脂肪酸的这些较低水平仅得到部分恢复。添加非必需脂肪酸16:0（5微摩尔）以及必需脂肪酸，导致缺乏脂肪酸的角质形成细胞中主要脂肪酸成功恢复正常。正常化的细胞在磷脂部分中显示出每毫克蛋白质的总脂肪酸含量恒定，因为随着脂肪酸补充量的增加，每个细胞的总细胞脂肪酸含量增加。在培养基中补充16:0和必需脂肪酸会降低细胞的生长和传代潜力。用18:1代替18:2脂肪酸会产生必需脂肪酸缺乏的角质形成细胞生长值。同样，补充最少的培养基（5微摩尔18:2 + 5微摩尔16:0）也给出了加速的细胞生长速率。这项研究表明，如果在生长培养基中伴有5微摩尔16:0，对必需脂肪酸水平的操纵会改变成人人类原代角质形成细胞的生长特性。
