Kuroda T, Natsuaki T, Wang W Q, Okuda S
Faculty of Agriculture, Utsunomiya University, Japan.
J Gen Virol. 1997 Apr;78 ( Pt 4):941-6. doi: 10.1099/0022-1317-78-4-941.
Double-stranded RNA multimers of cucumber mosaic virus (CMV) satellite RNA were detected in CMV-infected plants. RT-PCR showed that plus-sense and minus-sense monomers and plus-sense multimers of satellite RNA were present. Multimeric minus-sense RNA was not present except in the form of multimeric dsRNA. Sequence analysis of 52 cloned junction regions in head-to-tail repeats of unit-length satellite RNA indicated that about 35% of the junction sequences were precise fusions of monomer units, 56% lacked sequence of the 5' component, and 10% lacked sequence of both 3' and 5' components. No junction contained additional nucleotides. Deletions at the junction regions may have accumulated during CMV multiplication in inoculated plants. These data suggest that replicase is not released from the template during synthesis of multimeric molecules of satellite RNA.
在感染黄瓜花叶病毒（CMV）的植物中检测到了CMV卫星RNA的双链RNA多聚体。逆转录聚合酶链反应（RT-PCR）表明，卫星RNA的正链和负链单体以及正链多聚体均存在。除了多聚体双链RNA的形式外，不存在多聚体负链RNA。对单位长度卫星RNA首尾重复序列中52个克隆的连接区域进行序列分析表明，约35%的连接序列是单体单元的精确融合，56%缺少5'组分的序列，10%既缺少3'又缺少5'组分的序列。没有连接区域包含额外的核苷酸。在接种植物中CMV增殖过程中，连接区域的缺失可能已经积累。这些数据表明，在卫星RNA多聚体分子合成过程中，复制酶不会从模板上释放下来。
