Crook N E, James J D, Smith I R, Winstanley D
Horticulture Research International, Wellesbourne, Warwick, UK.
J Gen Virol. 1997 Apr;78 ( Pt 4):965-74. doi: 10.1099/0022-1317-78-4-965.
A cloned strain of Cydia pomonella granulovirus, CpGV-M1, was obtained using successive rounds of an in vivo limiting dilution method. A detailed physical map of the genome was constructed using 11 restriction enzymes. The region containing the granulin gene and an open reading frame immediately upstream of the granulin gene was sequenced. This region showed a high degree of homology to the equivalent region from Cryptophlebia leucotreta granulovirus with 98% amino acid identity for the granulins and 68% identity for the putative polypeptides encoded by the upstream ORFs. These latter polypeptides contained two zinc finger-like motifs and showed a low degree of homology to ME53 from Autographa californica nucleopolyhedrovirus (AcMNPV). Evidence is presented for a similar upstream ORF in Artogeia rapae GV also. Hybridization studies showed that the CpGV genome had a similar overall organization to the Artogeia rapae GV genome. Hybridization between CpGV and AcMNPV was limited to fragments spanning about 15% of each genome suggesting that very few genes are highly conserved between GVs and NPVs.
通过连续多轮体内有限稀释法获得了苹果蠹蛾颗粒体病毒的克隆株CpGV-M1。使用11种限制酶构建了该病毒基因组的详细物理图谱。对包含颗粒蛋白基因以及颗粒蛋白基因上游一个开放阅读框的区域进行了测序。该区域与苹果小卷蛾颗粒体病毒的对应区域显示出高度同源性，颗粒蛋白的氨基酸同一性为98%，上游开放阅读框编码的假定多肽的同一性为68%。这些后者的多肽含有两个锌指样基序，并且与苜蓿银纹夜蛾核多角体病毒（AcMNPV）的ME53显示出低度同源性。也有证据表明菜粉蝶颗粒体病毒中存在类似的上游开放阅读框。杂交研究表明，CpGV基因组与菜粉蝶颗粒体病毒基因组具有相似的总体结构。CpGV与AcMNPV之间的杂交仅限于跨越每个基因组约15%的片段，这表明颗粒体病毒和核多角体病毒之间极少有基因是高度保守的。
