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Physical map of the Cryptophlebia leucotreta granulosis virus genome and its relationship to the genome of Cydia pomonella granulosis virus.

作者信息

Jehle J A, Backhaus H, Fritsch E, Huber J

机构信息

Federal Biological Research Centre for Agriculture and Forestry, Institute of Biochemistry and Plant Virology, Germany.

出版信息

J Gen Virol. 1992 Jul;73 ( Pt 7):1621-6. doi: 10.1099/0022-1317-73-7-1621.

DOI:10.1099/0022-1317-73-7-1621
PMID:1629693
Abstract

A physical map of the genome of Cryptophlebia leucotreta granulosis virus (CIGV) was constructed for the restriction enzymes BamHI, EcoRI, KpnI, NdeI, NruI, SacI and XhoI using hybridization techniques. The size of the viral genome was determined to be 112.4 kbp. A restriction fragment library covering almost the entire genome of CIGV was constructed, and the position of the granulin gene was identified by cross-hybridization with granulin coding fragments of Cydia pomonella granulosis virus (CpGV). Two further regions of intergenomic similarity between CIGV and CpGV were mapped. These regions were aligned and show a collinear arrangement.

摘要

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