Terashima Y, Urist M R
Clin Orthop Relat Res. 1977(127):248-56.
Connective tissue outgrowths of muscle occur in vitro onto a substratum of bone matrix gelatin (BMG) chemically modified by various inorganic and organic cations or anions. Outgrowths onto BMG denatured by lyotropic concentrations of Ca2+ or complexed by only 1 mmole/1 of Cu2+ differentiate into fibrous tissue only. Onto BMG complexed with either positively or negatively charged organic molecules, the product was also fibrous tissue. Outgrowths onto undenatured and partially recalcified MBG differentiated into cartilage. These observations substantiate the view that differentiation of cartilage in tissue culture occurs in response to the undenatured form of a bone matrix protein molecular and not an unspecific surface net electrochemical charge.
肌肉的结缔组织生长物在体外形成于经各种无机和有机阳离子或阴离子化学修饰的骨基质明胶（BMG）基质上。在由溶致浓度的Ca2+变性或仅与1毫摩尔/升的Cu2+络合的BMG上的生长物仅分化为纤维组织。在与带正电或负电的有机分子络合的BMG上，产物也是纤维组织。在未变性和部分重新钙化的MBG上的生长物分化为软骨。这些观察结果证实了这样一种观点，即组织培养中软骨的分化是对骨基质蛋白分子的未变性形式而非非特异性表面净电化学电荷作出的反应。
