Urist M R, Nakagawa M, Nakata N, Nogami H
Arch Pathol Lab Med. 1978 Jun;102(6):312-6.
Bone matrix gelatin, prepared by chemical extraction of soluble noncollagenous proteins, was half digested with a chromatographically purified collagenase. The residue was placed on one side and autologous muscle on the other side of cellulose acetate membranes in diffusion chambers and tissue cultures. In this avascular system, the muscle septa connective tissue proliferated and differentiated into cartilage. Muscle tissue cultured in media conditioned with matrix residues and then transferred into a vascularized muscle pouch differentiated into cartilage and bone. These observations form the basis for a working hypothesis that myositis ossificans is a response of new populations of proliferating intramuscular connective tissue cells to a bone matrix-derived diffusible molecule.
通过化学提取可溶性非胶原蛋白制备的骨基质明胶，用经色谱纯化的胶原酶进行半消化处理。将残留物置于扩散室和组织培养中的醋酸纤维素膜一侧，自体肌肉置于另一侧。在这个无血管系统中，肌肉间隔结缔组织增殖并分化为软骨。在以基质残留物为条件的培养基中培养然后转移到血管化肌肉袋中的肌肉组织分化为软骨和骨。这些观察结果构成了一个工作假设的基础，即骨化性肌炎是增殖的肌内结缔组织细胞新群体对骨基质衍生的可扩散分子的反应。
