Peña C, Rodríguez de Lores Arnaiz G
Instituto de Química y Fisicoquimica Biológicas, Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina.
Neurochem Res. 1997 Apr;22(4):379-83. doi: 10.1023/a:1027343323629.
By means of a Sephadex G-50 column and anionic exchange HPLC a cerebral cortex soluble fraction (II-E) which highly inhibits neuronal Na(+)-K(+)-ATPase activity has been previously obtained. Herein, II-E properties are compared with those of the cardenolide ouabain, the selective and specific Na+, K(+)-ATPase inhibitor. It was observed that alkali treatment destroyed II-E but not ouabain inhibitory activity. II-E presented a maximal absorbance at 265 nm both at pH 7 and pH 2 which diminished at pH 10. Ouabain showed a maximum at 220 nm which was not altered by alkalinization. II-E was not retained in a C-18 column, indicating its hydrophilic nature, whereas ouabain presented a 26-min retention time in reverse phase HPLC. Therefore, it is concluded that the inhibitory factor present in II-E is structurally different to ouabain.
借助葡聚糖G - 50柱和阴离子交换高效液相色谱法，先前已获得一种能高度抑制神经元钠钾ATP酶活性的大脑皮质可溶性组分（II - E）。在此，将II - E的特性与强心苷哇巴因（一种选择性和特异性的钠钾ATP酶抑制剂）的特性进行比较。观察到碱处理会破坏II - E的活性，但不会破坏哇巴因的抑制活性。II - E在pH 7和pH 2时在265 nm处有最大吸光度，在pH 10时吸光度降低。哇巴因在220 nm处有最大值，碱化不会改变该值。II - E不保留在C - 18柱中，表明其具有亲水性，而哇巴因在反相高效液相色谱中的保留时间为26分钟。因此，得出结论，II - E中存在的抑制因子在结构上与哇巴因不同。
