Maggio B
Departamento de Química Biológica-CIQUIBIC, Facultad de Ciencias Químicas-CONICET, Universidad Nacional de Córdoba, Argentina.
Neurochem Res. 1997 Apr;22(4):475-81. doi: 10.1023/a:1027367929081.
The molecular organization, interactions, phase state and membrane-membrane interactions of model membranes containing cerebroside (GalCer), sulfatide (Sulf) and myelin basic protein (MBP) were investigated. Sulf shows a larger cross-sectional area than GalCer, in keeping with the lateral electrostatic repulsions in the negatively charged polar head group. The interactions of GalCer with different phospholipids are similar while those with Sulf depend on the phosphoryl choline moiety in the phospholipid. MBP induces a decrease of the phase transition temperature in both lipids but with Sulf this occurs at lower proportions of MBP. In mixtures of Sulf with phosphatidylcholine MBP induces phase separation among Sulf-rich and PC-rich domains. Extensive apposition of bilayers containing Sulf is induced by MBP while GalCer interferes with this process. Few membrane interactions proceed to bilayer merging or whole bilayer fusion and the glycosphingolipids help preserve the membrane integrity.
研究了含有脑苷脂（GalCer）、硫脂（Sulf）和髓鞘碱性蛋白（MBP）的模型膜的分子组织、相互作用、相态以及膜-膜相互作用。硫脂的横截面积比脑苷脂大，这与带负电荷的极性头部基团中的横向静电排斥作用一致。脑苷脂与不同磷脂的相互作用相似，而与硫脂的相互作用则取决于磷脂中的磷酰胆碱部分。MBP导致两种脂质的相变温度降低，但对于硫脂，这种情况在较低比例的MBP时发生。在硫脂与磷脂酰胆碱的混合物中，MBP诱导富含硫脂和富含PC的区域之间发生相分离。MBP诱导含有硫脂的双层膜广泛并列，而脑苷脂则干扰这一过程。很少有膜相互作用会导致双层膜合并或整个双层膜融合，糖鞘脂有助于维持膜的完整性。
