Patella V, de Crescenzo G, Marino I, Genovese A, Adt M, Gleich G J, Marone G
Division of Clinical Immunology and Allergy, University of Naples Federico II, Italy.
Int Arch Allergy Immunol. 1997 May-Jul;113(1-3):200-2. doi: 10.1159/000237546.
Eosinophilia in humans is associated with eosinophil infiltration and cardiac localization of eosinophil granule proteins. Eosinophil cationic proteins are responsible for cardiac disease in some patients with eosinophilia. We have investigated the in vitro effect of four eosinophil granule proteins: eosinophil cationic protein (ECP), major basic protein (MBP), eosinophil-derived neurotoxin (EDN) and eosinophil peroxidase (EPO), on mast cells isolated from human cardiac tissue (HHMC). ECP and, to a lesser extent MBP (0.3-3 microM), but not EDN and EPO, stimulated the release of histamine and tryptase from HHMC. This release reaction induced by ECP and MBP was Ca(2+)- and temperature-dependent and was abolished by preincubation with anti-ECP and anti-MBP, respectively. The activation of HHMC by ECP and MBP was abolished by preincubation with 2-deoxy-D-glucose and antimycin A. These data demonstrate that some eosinophil cationic proteins, ECP and MBP, are selective activators of HHMC, thus contributing to the cardiac lesions in patients with eosinophilia.
人类嗜酸性粒细胞增多与嗜酸性粒细胞浸润以及嗜酸性粒细胞颗粒蛋白在心脏的定位有关。在一些嗜酸性粒细胞增多的患者中，嗜酸性粒细胞阳离子蛋白是导致心脏疾病的原因。我们研究了四种嗜酸性粒细胞颗粒蛋白：嗜酸性粒细胞阳离子蛋白（ECP）、主要碱性蛋白（MBP）、嗜酸性粒细胞衍生神经毒素（EDN）和嗜酸性粒细胞过氧化物酶（EPO）对从人心脏组织分离的肥大细胞（HHMC）的体外作用。ECP以及程度较轻的MBP（0.3 - 3 microM），但不是EDN和EPO，刺激了HHMC释放组胺和类胰蛋白酶。由ECP和MBP诱导的这种释放反应是钙和温度依赖性的，并且分别通过与抗ECP和抗MBP预孵育而被消除。用2 - 脱氧 - D - 葡萄糖和抗霉素A预孵育可消除ECP和MBP对HHMC的激活作用。这些数据表明，一些嗜酸性粒细胞阳离子蛋白，如ECP和MBP，是HHMC的选择性激活剂，从而导致嗜酸性粒细胞增多患者的心脏病变。
