Okayama Y, el-Lati S G, Leiferman K M, Church M K
Immunopharmacology Group, Southampton General Hospital, England.
J Allergy Clin Immunol. 1994 May;93(5):900-9. doi: 10.1016/0091-6749(94)90384-0.
We have investigated the activity of the four principal cationic proteins of the eosinophil granules, major basic protein (MBP), eosinophil peroxidase (EPO), eosinophil-derived neurotoxin, and eosinophil cationic protein on histamine release from human skin mast cells. These four cationic proteins, over the concentration range of 10 to 200 micrograms/ml, did not induce significant histamine release, nor did they prime anti-IgE-induced histamine release from human skin mast cells significantly. However, a brief incubation (15 minutes) of two of the four principal eosinophil granule proteins, MBP and EPO, at concentrations of 50 to 200 micrograms/ml, caused a significant concentration-related inhibition of histamine release induced by 30 mumol/L substance P. The concentrations producing 50% inhibition for MBP and EPO on substance P-induced histamine release were 30 micrograms/ml and 100 micrograms/ml, respectively. This inhibitory effect appears to be a direct effect of these proteins on skin mast cells because purified (78% to 85%) skin mast cells displayed a similar response to MBP and EPO (n = 4). Also, when skin mast cells were incubated with 100 micrograms/ml MBP and EPO for 15 minutes and washed twice before activation by substance P, the inhibitory effect was not altered. These two proteins also inhibited histamine release induced by 10 micrograms/ml compound 48/80. These results suggest that MBP and EPO affect the same binding site(s) on skin mast cells as those of substance.
我们研究了嗜酸性粒细胞颗粒的四种主要阳离子蛋白，即主要碱性蛋白（MBP）、嗜酸性粒细胞过氧化物酶（EPO）、嗜酸性粒细胞衍生神经毒素和嗜酸性粒细胞阳离子蛋白对人皮肤肥大细胞组胺释放的作用。在10至200微克/毫升的浓度范围内，这四种阳离子蛋白均未诱导显著的组胺释放，也未显著增强抗IgE诱导的人皮肤肥大细胞组胺释放。然而，四种主要嗜酸性粒细胞颗粒蛋白中的两种，即MBP和EPO，在浓度为50至200微克/毫升时短暂孵育（15分钟），会对30微摩尔/升P物质诱导的组胺释放产生显著的浓度依赖性抑制。MBP和EPO对P物质诱导的组胺释放产生50%抑制的浓度分别为30微克/毫升和100微克/毫升。这种抑制作用似乎是这些蛋白对皮肤肥大细胞的直接作用，因为纯化的（78%至85%）皮肤肥大细胞对MBP和EPO表现出类似的反应（n = 4）。此外，当皮肤肥大细胞与100微克/毫升的MBP和EPO孵育15分钟并在被P物质激活前洗涤两次时，抑制作用未改变。这两种蛋白还抑制了10微克/毫升化合物48/80诱导的组胺释放。这些结果表明，MBP和EPO影响皮肤肥大细胞上与P物质相同的结合位点。
