Qian M, Johnson A E, Källström L, Carrer H, Södersten P
Department of Clinical Neuroscience, Karolinska Institute, Novum, Huddinge, Sweden.
Neuroscience. 1997 Apr;77(4):1077-89. doi: 10.1016/s0306-4522(96)00538-6.
Receptor autoradiography was used to investigate the distribution of brainstem binding sites for cholecystokinin, dopamine and N-methyl-D-aspartate with particular reference to the nucleus of the solitary tract of the rat, an area involved in the control of ingestive behavior. Binding sites for the A and B subtypes of the cholecystokinin receptor, labeled with [(125)I]cholecystokinin octapeptide sulfate in the presence or absence of antagonists for the devazepide (A) or L-365,260 (B) receptor, were present throughout the caudal rostral extent of the nucleus of the solitary tract, the A type predominating in the commissural, medial and gelatinous part and the B type in the lateral part. In the most rostral part of the medial nucleus of the solitary tract, both A and B receptors were present. Dopamine D2 receptors, labeled with [(125)I]NCQ-298, were found in all parts of the nucleus of the solitary tract. No binding to the dopamine D1 receptor, labeled with [(125)I]SCH-23982, was found in the brainstem. N-Methyl-D-aspartate receptors, labeled with [(3)H]dizocilpine maleate, were also present in the entire caudorostral extent of the nucleus of the solitary tract. Binding to cholecystokinin A receptors was co-distributed with [(125)I]NCQ-298 and [(3)H]dizocilpine maleate binding in the caudal and rostral parts of the nucleus of the solitary tract, and binding to cholecystokinin B receptors overlapped with [(125)I]NCQ-298 and [(3)H]dizocilpine maleate binding in the rostral nucleus of the solitary tract. These results are consistent with the hypothesis that cholecystokinin, dopamine and glutamate interact in the nucleus of the solitary tract in the control of ingestive behavior.
采用受体放射自显影术研究大鼠孤束核中胆囊收缩素、多巴胺和N-甲基-D-天冬氨酸脑干结合位点的分布，特别关注孤束核这一参与摄食行为控制的区域。在存在或不存在地伐西匹（A）或L-365,260（B）受体拮抗剂的情况下，用[（125）I]硫酸胆囊收缩素八肽标记的胆囊收缩素受体A和B亚型的结合位点，在孤束核的整个尾侧-嘴侧范围内均有存在，A亚型在连合部、内侧部和胶状部占主导，B亚型在外侧部占主导。在孤束核内侧核的最嘴侧部分，A和B受体均有存在。用[（125）I]NCQ-298标记的多巴胺D2受体在孤束核的所有部分均有发现。在脑干中未发现用[（125）I]SCH-23982标记的多巴胺D1受体的结合。用[（3）H]马来酸氯胺酮标记的N-甲基-D-天冬氨酸受体也存在于孤束核的整个尾侧-嘴侧范围内。在孤束核的尾侧和嘴侧部分，胆囊收缩素A受体的结合与[（125）I]NCQ-298和[（3）H]马来酸氯胺酮的结合共同分布，而在孤束核嘴侧核中，胆囊收缩素B受体的结合与[（125）I]NCQ-298和[（3）H]马来酸氯胺酮的结合重叠。这些结果与胆囊收缩素、多巴胺和谷氨酸在孤束核中相互作用以控制摄食行为的假说一致。
