Kang M, Mitomi H, Sada M, Tokumitsu Y, Takahashi Y, Igarashi M, Katsumata T, Okayasu I
Department of Gastroenterology, School of Medicine, Kitasato University, Kanagawa, Japan.
Am J Surg Pathol. 1997 Apr;21(4):417-23. doi: 10.1097/00000478-199704000-00007.
To investigate epithelial cell proliferation and oncoprotein expression of the serrated adenoma, a term that has been used synonymously with mixed hyperplastic and adenomatous polyp, immunohistochemical staining using polyclonal antibodies against Ki-67 and p53, and a Bcl-2 monoclonal antibody, was performed and the results compared with those in hyperplastic polyps and tubular adenomas. A total of 20 serrated adenomas all characterized by a serrated glandular pattern, contained immature goblet cells, upper crypt zone mitotic figures, and a few nucleoli within the epithelial cells. Twenty hyperplastic polyps and 20 tubular adenomas (all with low-grade dysplasia) were examined, and lesions that contained separate areas of hyperplastic and adenomatous glands were excluded. The Ki-67-positive rate in the middle zone of the crypts in serrated adenomas was significantly higher than in hyperplastic polyps but lower than in tubular adenomas; a similar tendency was also noted for the upper zone. Both serrated adenomas and hyperplastic polyps demonstrated Bcl-2-positive reactivity that was essentially limited to the lower crypt zone, while in contrast, involvement in tubular adenomas often extended to the middle zone. No p53 overexpression was found in any category. These results suggest that serrated adenomas may be committed to independent growth.
为研究锯齿状腺瘤（该术语与混合性增生性腺瘤息肉同义）的上皮细胞增殖及癌蛋白表达情况，采用抗Ki-67和p53的多克隆抗体以及Bcl-2单克隆抗体进行免疫组化染色，并将结果与增生性息肉和管状腺瘤的结果进行比较。总共20例锯齿状腺瘤均具有锯齿状腺管模式，含有未成熟杯状细胞、隐窝上部有丝分裂象以及上皮细胞内有少量核仁。检查了20例增生性息肉和20例管状腺瘤（均为低级别发育异常），并排除了含有增生性腺管和腺瘤性腺管分离区域的病变。锯齿状腺瘤隐窝中部的Ki-67阳性率显著高于增生性息肉，但低于管状腺瘤；隐窝上部也观察到类似趋势。锯齿状腺瘤和增生性息肉均显示Bcl-2阳性反应，主要局限于隐窝下部区域，而相比之下，管状腺瘤的累及范围常扩展至中部区域。在任何类别中均未发现p53过表达。这些结果表明锯齿状腺瘤可能倾向于独立生长。
