Takahashi Y
Department of Pediatrics, Yokohama City University School of Medicine.
Arerugi. 1996 Dec;45(12):1244-55.
Hypersensitivity resulting from the ingestion or inhalation of buckwheat allergen causes immediate manifestation of symptoms including urticaria, wheezing, dyspnea, anaphylactic shock, which is considered to be IgE-mediated type I hypersensitivity. Using sera of patients with buckwheat allergy the immuno-reactivity to purified buckwheat protein was investigated by enzyme-linked immunosorbent assay (ELISA) and immunoblotting method. The incidence of positive RAST value to buckwheat was 80% in the patients and 66.6% in the RAST-positive controls, whereas the RAST-negative controls showed all negative. The sera of both patients and RAST-positive controls showed significantly higher levels of specific IgG antibodies against salt-soluble and salt-insoluble fractions of buckwheat protein than the RAST-negative controls. By means of IgG-immunoblotting analysis, ten polypeptide bands of salt-soluble fraction and six polypeptides bands of salt-insoluble fraction were detected in all sera of the patients. In contrast, the patterns of IgE-immunoblots varied depending on the sera used. Taken together, the immune activation to buckwheat protein in patients with buckwheat allergy is not restricted to IgE antibody formation but extends to other immunoglobulin class. IgG, which indicating that the overall immune activation to buckwheat protein may be the basic characteristics of buckwheat allergy. Additionally, other factor is necessary for the manifestation of symptoms.
因摄入或吸入荞麦过敏原而导致的超敏反应会使症状立即显现，包括荨麻疹、喘息、呼吸困难、过敏性休克，这被认为是IgE介导的I型超敏反应。利用荞麦过敏患者的血清，通过酶联免疫吸附测定（ELISA）和免疫印迹法研究了对纯化荞麦蛋白的免疫反应性。患者中对荞麦的RAST阳性值发生率为80%，RAST阳性对照中为66.6%，而RAST阴性对照均为阴性。患者和RAST阳性对照的血清中针对荞麦蛋白盐溶性和盐不溶性部分的特异性IgG抗体水平均显著高于RAST阴性对照。通过IgG免疫印迹分析，在所有患者血清中检测到盐溶性部分的十条多肽带和盐不溶性部分的六条多肽带。相比之下，IgE免疫印迹的模式因所用血清而异。综上所述，荞麦过敏患者对荞麦蛋白的免疫激活不仅限于IgE抗体的形成，还扩展到其他免疫球蛋白类别。IgG表明对荞麦蛋白的整体免疫激活可能是荞麦过敏的基本特征。此外，症状的显现还需要其他因素。
