Yamada K, Urisu A, Kondou Y, Wada E, Komada H, Inagaki Y, Yamada M, Torii S
Department of Pediatrics, Fujita Health University School of Medicine.
Arerugi. 1993 Oct;42(10):1600-9.
Immediate hypersensitive reactions (IHR) induced by buckwheat ingestion are considered to be IgE-mediated. However we found 28 subjects without IHR to buckwheat ingestion out of 46 subjects who had positive RAST values for both buckwheat and rice antigens. The IHR-positive group showed significantly higher RAST values for buckwheat antigens (p < 0.01) but lower RAST values for rice antigens (p < 0.01) than did the IHR-negative group. RAST values for buckwheat and rice were significantly correlated with each other (p < 0.01) in the IHR-negative group, but not in the IHR-positive group. An effective dose-dependent inhibition was obtained in a RAST inhibition assay between homologous combinations of inhibitor and disc antigens such as rice and rice or buckwheat and buckwheat. The IHR-positive group showed no significant RAST inhibition between heterogeneous combinations of rice and buckwheat antigens. In contrast, the IHR-negative group showed a significant decrease in IgE binding even in the RAST inhibition assay between heterogenous combinations. These results led to the conclusion that there is cross-reactivity with IgE antibodies between buckwheat and rice and that IgE antibodies from IHR-negative subjects might recognize the epitopes on buckwheat antigens which cross react with rice antigens, whereas IgE antibodies from IHR-positive subjects might bind to buckwheat-specific epitopes.
因摄入荞麦引发的速发型超敏反应（IHR）被认为是由IgE介导的。然而，我们在46名对荞麦和大米抗原RAST检测均呈阳性的受试者中发现，有28名受试者摄入荞麦后未出现IHR。与IHR阴性组相比，IHR阳性组的荞麦抗原RAST值显著更高（p < 0.01），但大米抗原RAST值更低（p < 0.01）。在IHR阴性组中，荞麦和大米的RAST值彼此显著相关（p < 0.01），而在IHR阳性组中则不然。在抑制剂与盘状抗原的同源组合（如大米与大米或荞麦与荞麦）之间进行的RAST抑制试验中，获得了有效的剂量依赖性抑制。在大米和荞麦抗原的异源组合之间，IHR阳性组未表现出显著的RAST抑制。相反，即使在异源组合之间的RAST抑制试验中，IHR阴性组的IgE结合也显著降低。这些结果得出结论，荞麦和大米之间存在与IgE抗体的交叉反应，IHR阴性受试者的IgE抗体可能识别与大米抗原发生交叉反应的荞麦抗原表位，而IHR阳性受试者的IgE抗体可能与荞麦特异性表位结合。
