Burakova T, Marcus H, Canaan A, Dekel B, Shezen E, David M, Lubin I, Segal H, Yair R
Department of Immunology, The Weizmann Institute of Science, Rehovot, Israel.
Transplantation. 1997 Apr 27;63(8):1166-71. doi: 10.1097/00007890-199704270-00018.
We recently described a new approach that enables the generation of human/mouse chimera by adoptive transfer of human peripheral blood mononuclear cells into lethally irradiated normal strains of mice or rats, radioprotected with bone marrow from donors with severe combined immune deficiency. In such human/mouse chimera, a marked humoral response to recall antigens, as well as a significant primary response to keyhole limpet hemocyanin, has been generated.
In the present study, the organ distribution of the engrafted human cells in the human/mouse and human/rat chimera was investigated by immunohistochemistry.
Our results show that the T cells seem to be distributed throughout the reticular endothelial system, almost behaving like particles without any homing specificity. The B cells, however, can barely be found in internal organs, such as the liver or the pancreas, and are concentrated in the secondary lymphoid system (e.g., spleen, lymph node, and nonencapsulated lymphoid tissue). The B cells, together with the engrafted human T cells, form mixed lymphoid follicles.
The different homing patterns exhibited by the T and B lymphocytes indicate that the homing receptors on human B cells might be cross-reactive with their mouse counterparts, in contrast to the human T cells, which seem to be unable to interact with the mouse homing receptors. The presence of human B and T lymphocytes in close proximity to each other in the lymphoid tissues is in accordance with the ability of human/BALB radiation chimera to mount significant primary human antibody responses.
我们最近描述了一种新方法，通过将人外周血单个核细胞过继转移到经致死剂量照射的正常小鼠或大鼠品系中，并使用严重联合免疫缺陷供体的骨髓进行辐射防护，从而生成人/鼠嵌合体。在这种人/鼠嵌合体中，已产生了对回忆抗原的显著体液反应以及对钥孔戚血蓝蛋白的显著初次反应。
在本研究中，通过免疫组织化学研究了人/鼠和人/大鼠嵌合体中移植的人细胞的器官分布。
我们的结果表明，T细胞似乎分布于整个网状内皮系统，其行为几乎像没有任何归巢特异性的颗粒。然而，在内脏器官如肝脏或胰腺中几乎找不到B细胞，它们集中在二级淋巴系统（如脾脏、淋巴结和非包膜淋巴组织）。B细胞与移植的人T细胞一起形成混合淋巴滤泡。
T和B淋巴细胞表现出的不同归巢模式表明，人B细胞上的归巢受体可能与其小鼠对应物发生交叉反应，而人T细胞似乎无法与小鼠归巢受体相互作用。淋巴组织中彼此紧邻的人B和T淋巴细胞的存在与人类/BALB辐射嵌合体产生显著的人初次抗体反应的能力一致。
