Iamaroon A, Wallon U M, Overall C M, Diewert V M
Department of Odontology and Oral Pathology, Faculty of Dentistry, ChiangMai University, Thailand.
Arch Oral Biol. 1996 Dec;41(12):1109-19. doi: 10.1016/s0003-9969(96)00097-0.
Tissue remodelling is an important feature during embryogenesis. Although the matrix metalloproteinases are believed to participate in these processes, the relation between matrix metalloproteinases and tissue remodelling during craniofacial morphogenesis remains unclear. The purpose of the study was to look for the presence of enzymes involved in extracellular matrix degradation during craniofacial morphogenesis. Protein expression of the matrix metalloproteinase, 72-kDa gelatinase (matrix metalloproteinase-2, gelatinase A, 72-kDa type IV collagenase) was studied by gelatine zymography and by indirect immunofluorescence with conventional and confocal microscopy. In the anterior region of the developing mouse face, 72-kDa gelatinase was labelled mainly in the tips and peripheral regions of the nasal and facial prominences. Upon contact and fusion of the prominences, the staining was intensely localized to the zone of the fusion and the tips and peripheral regions of the nasal prominences and the maxilla. The labelling of 72-kDa gelatinase was also present in the peripheral regions of the mandible, second branchial arch, and the face around the developing eye. However, during lens vesicle formation, the staining of 72-kDa gelatinase was absent in the invaginated lens ectoderm. After the lens had completely detached from the surface ectoderm, the staining was resumed in the corneal epithelium and mesenchyme. Gelatine zymography was used to confirm the presence of active and latent 72-kDa gelatinase in the developing mouse craniofacial complex. Collectively, these data indicate that 72-kDa gelatinase may play a significant part in localized tissue remodelling during craniofacial morphogenesis and the aberrant expression or function of the enzyme could be involved in causing facial abnormalities.
组织重塑是胚胎发育过程中的一个重要特征。尽管基质金属蛋白酶被认为参与了这些过程，但在颅面形态发生过程中，基质金属蛋白酶与组织重塑之间的关系仍不清楚。本研究的目的是寻找在颅面形态发生过程中参与细胞外基质降解的酶的存在情况。通过明胶酶谱法以及使用传统显微镜和共聚焦显微镜的间接免疫荧光法，研究了基质金属蛋白酶72-kDa明胶酶（基质金属蛋白酶-2、明胶酶A、72-kDa IV型胶原酶）的蛋白表达。在发育中小鼠面部的前部区域，72-kDa明胶酶主要标记在鼻和面部隆起的尖端及周边区域。当隆起接触并融合时，染色强烈定位于融合区域以及鼻隆起和上颌的尖端及周边区域。72-kDa明胶酶的标记也存在于下颌骨、第二鳃弓以及发育中眼睛周围面部的周边区域。然而，在晶状体泡形成过程中，72-kDa明胶酶在凹陷的晶状体外胚层中无染色。晶状体完全从表面外胚层分离后，角膜上皮和间充质中恢复染色。明胶酶谱法用于证实发育中小鼠颅面复合体中存在活性和潜伏性72-kDa明胶酶。总体而言，这些数据表明72-kDa明胶酶可能在颅面形态发生过程中的局部组织重塑中起重要作用，该酶的异常表达或功能可能与面部异常的发生有关。
