垂体腺苷酸环化酶激活多肽和一氧化氮合酶在大鼠睫状神经节中表达。
Pituitary adenylate cyclase activating polypeptide and nitric oxide synthase are expressed in the rat ciliary ganglion.
作者信息
Elsäs T, Uddman R, Mulder H, Sundler F
机构信息
Department of Ophthalmology, University Hospital of Trondheim, Norway.
出版信息
Br J Ophthalmol. 1997 Mar;81(3):223-7. doi: 10.1136/bjo.81.3.223.
AIMS
To study the distribution in the rat ciliary ganglion of neurons synthesising and storing the recently discovered neuropeptide pituitary adenylate cyclase activating polypeptide (PACAP) and neuronal nitric oxide synthase (NOS), the neuronal marker of the novel gaseous transmitter nitric oxide.
METHODS
Neurons expressing PACAP and neuronal NOS mRNA were identified in the rat ciliary ganglion by in situ hybridisation with radiolabelled oligonucleotide probes. Immunocytochemistry was used to demonstrate immunoreactive neuropeptides and NOS.
RESULTS
Immunocytochemistry demonstrated immunoreactivity for PACAP and NOS in a small number of neuronal cell bodies. In situ hybridisation revealed that NOS and PACAP were expressed in numerous ganglion cell somata. The well established ciliary messengers vasoactive intestinal peptide and neuropeptide Y were found in a large number of neuronal cell bodies.
CONCLUSION
These results demonstrate that PACAP and NOS are synthesised and stored in the ciliary ganglion. These findings further illustrate the mixed nature of the ciliary ganglion and may provide a basis for the understanding of the diverse physiological functions of this ganglion.
目的
研究合成并储存最近发现的神经肽垂体腺苷酸环化酶激活多肽（PACAP）以及神经元型一氧化氮合酶（NOS，新型气体递质一氧化氮的神经元标志物）的神经元在大鼠睫状神经节中的分布情况。
方法
通过用放射性标记的寡核苷酸探针进行原位杂交，在大鼠睫状神经节中鉴定出表达PACAP和神经元型NOS信使核糖核酸的神经元。采用免疫细胞化学方法来显示免疫反应性神经肽和NOS。
结果
免疫细胞化学显示少数神经元细胞体对PACAP和NOS有免疫反应性。原位杂交表明NOS和PACAP在众多神经节细胞胞体中表达。在大量神经元细胞体中发现了成熟的睫状信使血管活性肠肽和神经肽Y。
结论
这些结果表明PACAP和NOS在睫状神经节中合成并储存。这些发现进一步说明了睫状神经节的混合性质，并可能为理解该神经节的多种生理功能提供基础。
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